Expression of Heat Shock Protein After Ischemic Preconditioning in Rabbit Hearts
スポンサーリンク
概要
- 論文の詳細を見る
Previous studies have shown that preconditioning(PC)with a brief ischemic episode induces heat shock protein(HSP)in cardiac tissue. However, it is unclear when and where in the left ventricle HSP is expressed after PC. Hence, the expression of HSP was studied in rabbit hearts at various time intervals after PC using immunohistochemical methods. Rabbits were preconditioned four times with 5 min of occlusion and 5 min of reperfusion of the coronary artery and then were killed at 0, 3, 24, 48, 72 and 168h after the PC(n=4, for each time interval). Samples were obtained from the subendocardium and subepicardium of the preconditioned and nonpreconditioned wall and these were processed to 4 μm thick cryosections. The sections were immunolabelled with mouse monoclonal IgGs against HSP 72/73. Positive immunoreactivity was observed as early as 3h after PC, persisting up to 72h but not detected at 168h. HSP was expressed not only in the preconditioned myocardium but also in the remote nonpreconditioned myocardium. There was a wide variation in expression among myocytes. Expression was dominant in myocytes compared with vessel walls. It was concluded that PC induced transient and inhomogeneous expression of HSP in rabbit hearts.
- 社団法人日本循環器学会の論文
- 1998-06-20
著者
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Fujiwara Takako
Kyoto Women's University
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Takahashi Masaaki
The Third Division Department Of Internal Medicine Kyoto University
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Sasayama S
Department Of Life And Medical Science Doshisha University Kyotanabe & Daijukai Hospital
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Sasayama Shigetake
浜松労災病院
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Ohtani Seiji
The Third Division Of Medicine Kyoto University
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FUJIWARA HISAYOSHI
The Third Division,Department of Internal Medicine, Faculty of Medicine, Kyoto University
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Sasayama Shigetake
The Department Of Cardiology Hyogo Prefectural Amagasaki Hospital
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Tanaka M
Osaka City Univ. Medical School
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Tanaka Masahiro
Rakuwakai Otowa Hospital
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SASAYAMA SHIGETAKE
The Third Division,Department of Internal Medicine,Kyoto University Hospital
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Sasayama Shigetake
The Third Division Of Medicine Kyoto University
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Sasayama Shigetake
Hamamatsu Rosai Hospital
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Inada Tsukasa
The Third Division Of Medicine Kyoto University
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Fujiwara Takako
Kyoto Women's University
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Fujiwara Hisayoshi
The Third Division Of Medicine Kyoto University
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Tanaka Masaru
The Third Division of Medicine, Kyoto University
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Yamasaki Kenzo
The Third Division of Medicine, Kyoto University
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Yokota Ryoji
The Third Division of Medicine, Kyoto University
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Doyama Kiyoshi
The Third Division of Medicine, Kyoto University
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Sawada Shigeo
Virus Center National Kyoto Hospital
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Sasayama Shigetake
Department Of Cardiovascular Medicine Graduate School Of Medicine Kyoto University
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Fujiwara Hisayoshi
The Third Division Of Internal Medicine Kyoto University
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Sasayama Shigetake
The Third Division Department Of Internal Medicine And Central Clinical Laboratory Kyoto University
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Sasayama Shigetake
Division Of Medicine Hamamatsu Rosai Hospital Shizuoka
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Yokota Ryoji
The Third Division Of Medicine Kyoto University
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Doyama Kiyoshi
The Third Division Of Medicine Kyoto University
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Yamasaki Kenzo
The Third Division Of Medicine Kyoto University
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Sasayama Shigetake
Department Of Cardiology Doshisha University
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Sasayama Shigetake
The Third Division Department Of Internal Medicine Faculty Of Medicine Kyoto University
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Ono Shinji
Third Division Department Of Internal Medicine Faculty Of Medicine Kyoto University
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Takashima Masayuki
Third Department Of Internal Medicine Kyoto University Faculty Of Medicine
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Tanaka Masaru
Department Of Cardiology Cardiovascular Center Osaka Red Cross Hospital
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Tanaka Masaru
The Third Division Of Medicine Kyoto University
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Sasayama Shigetake
Third Division Department Of Internal Medicine Kyoto University
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Tanaka Makoto
Cardiovascular Center Osaka Red Cross Hospital
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Fujiwara Hisayoshi
The Third Division Department Of Internal Medicine Faculty Of Medicine Kyoto University Kyoto
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Sasayama Shigetake
Second Division Internal Medicine Toyama Medical And Pharmaceutical School
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Sasayama Shigetake
Thesecond Department Of Internal Medicine Toyama Medical And Pharmaceutical University
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Tanaka Masaru
Third Division Department Of Internal Medicine Kyoto University
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Takashima Masayuki
Cardiovascular Center Osaka Red Cross Hospital
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