ISSRマーカーによるハナハス品種の親子鑑定と花粉親の推定(育種・遺伝資源)

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Four intraspecific hybrids of Nelumbo nucifera, 3 interspecific hybrids from N. nucifera and N. lutea, 69 N. nucifera and 1 N, lutea cultivars were used for the identification of parent-offspring relationships by the ISSR method. Eleven ISSR primers amplified 103 reproducible polymorphic bands. Fifty-eight polymorphic bands were amplified from N. nucifera and could be useful for the identification of cultivars. It suggested that there were significant differences between N. nucifera and N. lutea. With these marks, 16 and 81 polymorphic bands were amplified from artificial hybrid progeny of N. nucifera (Zhongriyouyilian), artificial interspecific hybrid from N. nucifera and N. lutea (Maihiren) and their maternal cultivars, respectively. The relationship among the cultivars followed the genetic principle. However, neither hypothesis of the genetic relationship of 'Byakkohren' originating from (Shirokunshishohren × Kourinren) or (America - byakuren × Seigetsuren) was verified. However, 12, 4, 13 and 16 polymorphic bands respectively were amplified from natural hybrids of N. nucifera (Fenwanlian and Meihongchuantai), natural interspecific hybrids from N. nucifera and N. lutea (Meizhonghong and Xiaowufei) and their maternal cultivars. And from the patterns of 69 paternal candidates, only 4 cultivars (Shouxingtao, Rozanpakuren, Shouxingtao and Fenqianye, respectively) fitted genetically and could be reasonably regarded as possible parental plants. These results suggested that ISSR was a useful method for identification of parent-offspring relationship and presumption of pollen parent in lotus.
園芸学会の論文
2004-09-15

ISSRマーカーによるハナハス品種の親子鑑定と花粉親の推定(育種・遺伝資源)

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