マウス多臓器アルカリSCG法による臓器特異的遺伝子毒性の検出

概要

論文の詳細を見る
The genotoxicity of 54 chemicals, selected from IARC(International Agency for Research on Cancer)Group 1, 2A, or 2B, on mouse organs was determined by the alkaline single cell gel electrophoresis(SCG)assay. Groups of four mice were treated once orally or intraperitoneally, and stomach, colon, liver, kidney, bladder, lung, brain, and/or bone marrow were sampled 3, 8, and/or 24h after treatment. The positive rate was 96.3%;only two non-mutagenic carcinogens(benzenehexachloride and carbon tetrachloride)were negative. Concordance between genotoxicity and carcinogenicity(in mice and/or rats)in target organs was 67.3%, 53.8%, and 41.9% for liver, stomach, and lung, respectively. At≧8h, however, concordance was 83.9%, 73.3%, and 65.0% for the same three organs. These results suggest that persistence of DNA lesions can play an important role in the target organ specificity of certain carcinogens. Our present approach confirms that the alkaline SCG assay can be used to detect carcinogens in many organs concurrently.
日本環境変異原学会の論文
1998-04-10