大腸菌WP2uvrA株を用いた突然変異スペクトル解析系の開発

概要

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Two sets of useful tester strains(WP3101-WP3106 and WP3101P-WP3106P)derived from E.coli WP2uvrA were constructed for the detection and classification of mutagens. Six kinds of F' plasmid(lacI^-, lacZ^-, proAB^+)in strains WP3101-WP3106 carried a different lacZ^- allele. Assays for transitions and transversions are based upon Lac^+ reversion of a specific mutation located within the lacZ^- gene on an F' plasmid in the strains. Plasmid pKM101 was further transferred to each strain to make a set of strains WP3101P-WP3106P. In any strain the trpE65(ochre)allele is available for the Trp^+ reversion assays which is commonly used for mutagenicity test with the WP2uvrA strain. Using the new tester strains, the mutational specificities of 1, 2-dibromo-3-chloropropane and cumene hydroperoxide were investigated. In the presence of S9mix, 1, 2-dibromo-3-chloropropane specifically induced G:C→A:T transitions. Cumene hydroperoxide induced two types of transversions, G:C→T:A detected in WP3104 and A:T→T:A detected in WP3105, with G:C→T:A predominating. In pKM101-carrying strains, all 6 types of base substitutions were induced by cumene hydroperoxide with different efficiencies. The frequency of predominant G:C→T:A transversions was not affected by the introduction of pKM101, while that of other types of transitions and transversions were markedly increased.
日本環境変異原学会の論文
1998-07-21