粗タンパク直接固相法によるタンパク質プロファイリングシステムの開発

概要

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Proteome research in the medical field is expected to accelerate the understanding of disease mechanism, and to create new therapeutic and diagnostic concept. For protein profiling, this paper proposes a new methodology namaed CPDIB (Crude Protein Direct Blotting). In CPDIB assay, crude protein sample is directly immobilized on a hydrophobic membrane, and then the experssion molecules in the sample are analyzed quantitatively by using a special device, where the membrane is used as a field of immune reaction. The over-all structure of the special device is based on the conventional slot-blot device. Mechanical improvement in the air-tightness of the case holding the membrane realizes the direct blotting and results in the high performance of the stability in the immune reaction. This paper shows the mechanical strucutre of the membrance aasembly called ImmobiChip and the evaluation of the protein profiling and its reproducibility.
社団法人精密工学会の論文
2003-12-05