Evaluation of an Automatic Device to Prepare Fibronectin Eyedrops Using a Patient's Own Plasma

概要

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Fibronectin (FN) has been reported to be effective for the treatment of corneal trophic ulcers. but the FN eyedrop preparation is not a simple technique. We previously developed an automatic device to prepare FN eyedrops using a patient's own plasnla (Yoshida et al. Jpn. J. Hosp. pharm., 24. 493-498 ( 1998)). The aim of' this study was to evaluate the efficiency of this automatic device. FN eyedrops were prepared using plasma from healthy individuals either with an automttic device or a manual process using gelatin-coupled Sepharose 4 B (GS 4 B) and Sephadex G-25 (PD-10). The average FN concentration and the recovery rate in the eyedrops prepared using the automatic device were 653±57μg per ml and 55±l5%, respectively. but those prepared using the manual process were 563±50μg per ml and 46±6%. respectively. The operation of the device was easy and the recovery values of FN were similar among all operators. The total preparation time of FN eyedrops was approximately I hour. This automatic de-vice was thus found to be useful for preparing FN eyedrops from the patient's own plasma.
日本医療薬学会の論文
1999-08-10