Comparative Study of Autologous Fibrin Glues Prepared by Cryo-Centrifugation, Cryo-Filtration, and Ethanol Precipitation Methods
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概要
- 論文の詳細を見る
To establish a speedy preparation method for the fibrinogen-rich fraction (FRF) from autologous plasma using fibrin glue, we compared the concentrations and yields of coagulation factors in FRF prepared by 3 methods. Human plasma from healthy volunteers was divided into 3 samples. Two samples were frozen at -20℃ in a freezer and defrosted in a 4℃ water bath. One sample of defrosted plasma was centrifuged and FRF was obtained (C method). Another sample of defrosted plasma was filtered and FRF was obtained (F method). The last sample was treated with cold ethanol(1/10) in a 4℃ water bath and FRF was obtained after centrifugation (E method). The concentrations of fibrinogen, fibronectin, factor XIII, and plasminogen in each obtained FRF were measured and yields were calculated. (1) The volume of FRF obtained by the E method was greater than that by the C method, but less than that by the F method. While the variation in volume obtained by the E method was the lowest among the 3 methods; (2) the concentrations of fibrinogen obtained by the E and C method were similar, but the yield from the E method was the highest; (3) the concentration and yield of fibronectin from the E and C method were similar and were greater than those by the F method; (4) the concentration and yield of factor XIII from the E method were significantly higher than those from the other methods; (5) the E method preparation time was about 1 h, the shortest among the 3 methods. These results indicate that high quality FRF from autologous plasma can be prepared easily and within 1 h by the E method.
- 社団法人日本薬学会の論文
- 1999-11-15
著者
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KAMIYA Akira
Department of Pharmacy, Yamaguchi University Hospital
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Kamiya A
Department Of Pharmacy Yamaguchi University Hospital
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Kamiya Akira
Department Of Pharmacy Yamaguchi University Hospital
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YOSHIDA Hisahiro
Department of Drug Metabolism and Disposition, Meiji Pharmaceutical University
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Yoshida H
Department Of Pharmacy Yamaguchi University Hospital
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Yoshida H
Yamaguchi Univ. Hospital Yamaguchi Jpn
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Yoshida Hisahiro
Department Of Drug Metabolism And Disposition Meiji Pharmaceutical University
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HIROZANE Kiyoshi
Department of Pharmacy, Yamaguchi University Hospital
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Kamiya Akira
Department Of Biomedical Engineering University Of Tokyo
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Hirozane Kiyoshi
Department Of Pharmacy Yamaguchi University Hospital
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