Polymorphism in the acetylcholinesterase gene of the housefly, Musca domestica L. (Diptera:Muscidae)

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概要

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• The cDNA fragments of a 2kb gene, encoding the housefly acetylcholinesterase (AChE) were compared among 6 strains. While there were 4 substitutions of amino acids in the predicted peptides between Asp_<83> and Arg_<648> which is well conserved in insects, the following contrasts were made among the strains. The insecticide susceptible strains, aabys and SRS had Gly_<342> and Phe_<407> and the resistant strains, LPR, YBOL and YPRN had Ala_<342> and Tyr_<407> with Val_<342> unique to YBOL. In addition, Leu_<260> was found only in the chiral organophosphate resistant strains, YBOL and YPRN. These results showed that the patterns of single nucleotide polymorphisms, which resulted in amino acid substitutions, can be a practical tool for characterization of the insecticide resistance of houseflies.

• 日本応用動物昆虫学会の論文
• 2001-08-25

著者

• Tomita Takashi

  National Inst. Of Infectious Diseases Tokyo

• KONO Yoshiaki

  Department of Medical Entomology, National Institute of Infectious Diseases

• Shono Toshio

  Institute of Agriculture and Forestry, The University of Tsukuba

• TOMITA Takashi

  Department of Pharmacy, Tsukuba University Hospital

• Shono Toshio

  Institnte Of Agriculture And Forestry University Of Tsukuba

• Kono Y

  Mitsubishi Heavy Industries Ltd. Kobe Shipyard & Machinery Works

• Tomita Takashi

  Department Of Medical Entomology National Institute Of Infectious Diseases

• Kono Y

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Kozaki Toshinori

  Institute of Agriculture and Forestry, University of Tsukuba

• KOZAKI Toshinori

  Department of Medical Entomology, National Institute of Infectious Diseases

• Kozaki Toshinori

  Department Of Medical Entomology National Institute Of Infectious Diseases

• Kono Yoshiaki

  Department Of Educational Psychology Tokyo Gakugei University

• Tomita Takashi

  Department Of Electric And Electronic Engineering Toyohashi University Of Technology

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