Characterization of the Activity of L-Ascorbic Acid 2-[3,4-Dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6-yl-hydrogen Phosphate] Potassium Salt in Hydroxyl Radical Elimination

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The effect of L-ascorbic acid 2-[3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6-yl-hydrogen phosphate] potassium salt (EPC-K_1) on hydroxyl radical (・OH) elimination was studied using electron spin resonance (ESR) and spectrophotometric experiments. The addition of EPC-K_1 and ・OH scavengers eliminated the ・OH generated from Cu^<2+>/H_2O_2,Fe^<2+>/H_2O_2 and H_2/UV-irradiation reaction systems. However, in competitive reactions using different concentrations of a spin-trap agent, the addition of the ・OH scavenger altered the IC_<50> values, whereas the addition of EPC-K_1 and a metal chelater did not change the value in the Cu^<2+>/H_2O_2 and Fe^<2+>/H_2O_2 reaction systems. The addition of EPC-K_1 and metal chelater changed the ESR signal for free Cu^<2+>. The spectrophotometric experiments confirmed that the addition of EPC-K_1 and metal chelater altered the absorption spectra due to CuCl_2 and FeSO_4,whereas the ・OH scavenger did not alter the spectra.Therefore, it was demonstrated that EPC-K_1 has the ability both to scavenge ・OH directly and to inhibit the generation of ・OH by the chelation of Cu^<2+> and Fe^<2+>.
公益社団法人日本薬学会の論文
2000-03-01