Expression of two acetylcholinesterase genes from organophosphate sensitive- and insensitive-houseflies, Musca domestica L. (Diptera: Muscidae), using a baculovirus insect cell system

概要

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Two types of housefly acetylcholinesterases (AChEs) were expressed in a baculovirus lepidopteran cell system to elucidate the effect of point mutations from Ile_<162>, Gly_<342> and Phe_<407> in the organophosphate susceptible strains to Met_<162>, Ala_<342> and Tnyr_<407> in the organophosphate resistant strains. Concentrations resulting in 50% inhibition (I_<50>) of the expressed enzyme by an organophosphate, fenitroxon, with Ile_<162>, Gly_<342> and Phe_<407> was 4.02 × 10^<-7>M, and that of the AChE with Met_<162>, Ala_<342> and Tyr_<407> was 9.00 × 10^<-6> M. The ratio of I_<50> values was 22.4, and was almost equivalent to the ratio of the inhibition assay of homogenates of housefly bodies from which the cDNAs encoding the housefly AChEs were derived. The insensitivity of the housefly AChE to fenitroxon in the insecticide resistant strains is attributed to point mutations in the structural gene.
日本応用動物昆虫学会の論文
2002-02-25