Effects of Catechins on Superoxide and Hydroxyl Radical

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Superoxide (O^-_2・) was reduced by the addition of superoxide dismutase (SOD : O^-_2・scavenger) and catechins. In competitive reactions utilizing different concentrations of spin-trap agent, the IC_<50> values of each sample were changed. With regard to the Cu^<2+>/H_2O_2 and Fe^<2+>/H_2O_2 reaction systems, metal chelater, hydroxyl radical (・OH) scavenger and catechins eliminated the levels of・OH. For the Cu^<2+>/H_2O_2 reaction systems, the IC_<50> for・OH scavenger changed, but that for metal chelater and catechins did not. However, for the Fe^<2+>/H_2O_2 reaction system, the IC_<50> for・OH scavenger and catechins changed, whereas that for metal chelater did not. The ESR signal for free Cu^<2+> was changed by addition of metal chelater and catechins. In hte spectrophotometer experiments, it was confirmed that the CuCl_2 spectrum was changed by addition of metal chelater and catechins but not by・OH scavenger. Conversely, the FeSO_4 spectrum was not changed by addition of・OH scavenger or catechins, but was altered by metal chelater. Lipid peroxidation was inhibited by catechins in a concentration-dependent manner.Therefore, it was suggested that the catechins did not scavenge directly the generated・OH from the Cu^<2+>/H_2O_2 reaction system, but inhibited the generation of・OH by acting on the Cu^<2+>/H_2O_2 reaction system. On the other hand, with respect to the・OH generated from the Fe^<2+>/H_2O_2 reaction system, it was suggested that the catechins had a direct scavenging capacity of the・OH, but had little chelating activity of iron.It was confirmed that catechins have the ability to scavenge for O^-_2・as well as・OH and to inhibit the generation of・OH by chelation with metal ions.
公益社団法人日本薬学会の論文
1999-02-15

Effects of Catechins on Superoxide and Hydroxyl Radical

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