Stimulatory Effects of Hydroxyl Radical Generation by Ga-Al-As Laser Irradiation on Mineralization Ability of Human Dental Pulp Cells(Molecular and Cell Biology)
スポンサーリンク
概要
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The present study was conducted to investigate the effects of Ga-Al-As laser irradiation on the mineralization ability of human dental pulp (HDP) cells. HDP cells in vitro were irradiated once with a Ga-AL-As laser at 0.5W for 500s and at 1.0W for 500s in order to investigate free radicals as one mechanism for transmission of laser photochemical energy to cells. Production of the hydroxyl radical (・OH) was measured using the ESR spintrapping method and was found to be increased by laser irradiation. The DMPO-OH was not detected in the presence of dimethyl sulfoxide (DMSO), a ・OH scavenger. The formation of calcification nodule was also investigated by von Kossa staining. The number of calcified nodules was increased by 1.0W-laser irradiation. Alkaline phosphatase (ALP) activity was higher in the 1.0W-laser irradiation group. Expression of mRNAs for heat shock protein 27, bone morphogenetic proteins (BMPs) and ALP were greater in the 1.0W-laser irradiation group. Expression of BMPs in the conditioned medium was also higher in the 1.0W-laser irradiation group. In particular, DMSO decreased the number of calcified nodule produced by 1.0W-laser irradiation. These results supposed that the mineralization of HDP cells is stimulated by laser irradiation, and that ・OH generated by laser irradiation is a trigger for promotion of HDP cell mineralization.
- 公益社団法人日本薬学会の論文
- 2007-01-01
著者
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Matsushima Kiyoshi
Department of Endodontics, Nihon University School Dentistry at Matsudo
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TSUJIMOTO Yasuhisa
Department of Endodontics, Nihon University School of Dentistry at Matsudo
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Matsui Satoshi
Department Of Endodontics Nihon University School Of Dentistry At Matsudo
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Matsushima Kiyoshi
Department Of Endodontics And Research Institute Of Oral Science Nihon University School Of Dentistr
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Tsujimoto Y
Department Of Endodontics Nihon University School Of Dentistry At Matsudo
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Tsujimoto Yasuhisa
Department Of Endodontics Nihon University School Of Dentistry At Matsudo
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