Effects of IL-6 and 1L-1β on MMP Activities in Fibreblastic Cells Derived from Human Dental Pulp and Gingiva

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In order to find functional characteristics of human dental pulp in the irreversible progress of pulpitis, we examined the effects of IL-1β and IL-6 on matrix metalloprotease (MMP) activity in human-dental-pulp derived fibroblastic cells (HDP) compared with human gingival-derived fibroblasts (HGF). The activities of MMPs activated by IL-1β or IL-6 in HDP and HGF were investigated with zymography. MMP-2 activity in conditioned media of both cells cultured with IL-1β or IL-6 were activated, but MMP-9 activity was not activated without plasminogen. When plasminogen was added to the conditioned medium, plasmin and MMP-9 were activated. Plasmin and MMP-9 of HDP-treated IL-6 were more activated than HGF. Next, we examined the effect of these cytokines on the plasminogen activator(PA)/plasmin system. The PA activity in the conditioned medium showed no difference when both cells were incubated with IL-1β. In contrast, when both cells were incubated with IL-6, the PA activity of HDP was higher than that of HGF. By reverse-transcription polymerase chain reaction (RT-PCR), tPA mRNA levels in both cells were enhanced by IL-1β and IL-6, but tPA was not effected. IL-6-stimulated tPA mRNA level of HDP was higher than that of HGF. These findings suggest that IL-6 may play a major role in extracellular matrix (ECM) degradation through stimulation of the PA/plasmin system in HDP of pulpitis.
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