Stimulation of Alkaline Phosphatase Activity by PGE_2 through Induction of IGF-1 in Human Dental Pulp Cells
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概要
- 論文の詳細を見る
Prostaglandin E_2 (PGE_2), which is considered to be an important local factor in bone metabolism, stimulates alkaline phosphatase (ALP) activity in dental pulp (HDP) cells at lower concentrations. Moreover, insulin-like growth factors (IGF_S) are widely expressed abundant autocrine and paracrine factors that regulate the proliferation and differentiation of various cells. PGE_2 is also a potent stimulator of IGF_S synthesis in bone formation. In this study, we examined the effect of PGE_2 on IGF induction in HDP cells. PGE_2 increased IGF-1 and IGFBP-5 mRNA level and production as compared to the control, and IGF-1 and IGFBP-5 significantly increased the ALP activities in a dose-dependent manner. On the other hand, the addition of the anti-IGF-1 antibody decreased ALP activity by PGE_2. These findings suggest that PGE_2 stimulates the formation of tertiary dentin through induction of IGF-1 and IGFBP-5 expressions in HDP cells.
- 日本大学の論文
著者
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Matsushima Kiyoshi
Department Of Endodontics And Research Institute Of Oral Science Nihon University School Of Dentistr
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Yamazaki Muneyoshi
Department Of Endodontics Nihon University School Of Dentistry At Matsudo
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Sakamoto Maki
Department of Endodontics, Nihon University School of Dentistry at Matsudo
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Sakamoto Maki
Department Of Endodontics Nihon University School Of Dentistry At Matsudo
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Matsushima Kiyoshi
Department of Endodontics
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