<Originals>Mechanisms of retinoid resistance in a myeloid cell line, YM711,transfected with PML/RARα cDNA : possible involvement of redox potential
スポンサーリンク
概要
- 論文の詳細を見る
We established a myeloid cell line (YM711) by transfecting exogenous PML/RARα cDNA into peripheral blood stem cells. The established cells were positive for CD33,CD34,CD38,CD13,CD14,and CD11b. Cytochemical examination also revealed YM711 cells to be positive for peroxidase, α-naphtyl butyrate esterase, and acid phosphatase. Although PML/ RARα mRNA was detected by reverse-transcription and polymerase chain reaction (RT-PCR) in YM711 cells, all-trans retinoic acid (ATRA) did not induce differentiation of these cells. We, therefore, designated the YM711 as being ATRA resistant. Because YM711 expressed multi-drug resistance 1 (MDR-1) mRNA and cell surface p-glycoprotein, we assessed whether verapamil and ATRA would induce the differentiation of YM711 and found not. Increased expression of cellular retinoic acid-binding protein-II (CRABP-II) was also detected on YM711 cells. We tried to suppress the expression of CRABP-II mRNA in vitro by using antisense-Soligonucleotides for CRABP-II mRNA. However differentiation of YM711 was not observed in the presence of 10^<-5> M ATRA. To induce differentiation of this cell line, we then focused on redox regulation. We used thiol compounds-free RPMI 1640 medium, and mixed this with regular RPMI 1640 at the ratio of 3 : 7. After 4 days in culture with the 3 : 7 medium, the expression of CD14 on YM711 cells was enhanced significantly following the treatment with 10^<-6> M ATRA. In conclusion, we suggest that redox status is an important factor for ATRA-induced differentiation of the ATRA-resistant cell line. Furthermore, this cell line may be useful in elucidating the mechanism of resistance of leukemia cells to retinoids.
- 近畿大学の論文
著者
-
Maeda Yasuhiro
Third Department Of Internal Medicine Kinki University School Of Medicine
-
Maeda Yasuhiro
近畿大学 第3内科
-
YAMAMURA Masaomi
Nagasaki CML Study Group
-
ANDO Kiyoshi
Tokai University
-
Kanamaru A
Department Of Hematology Kinki University School Of Medicine
-
Maeda Yasuhiro
Department Of Hematology Nephrology And Clinical Immunology Kinki University School Of Medicine
-
Matsuda M
Third Department Of Internal Medicine Kinki University School Of Medicine
-
KANAMARU Akihisa
Third Department of Internal Medicine, Kinki University School of Medicine
-
SUMIMOTO Yoshiyasu
Third Department of Internal Medicine, Kinki University School of Medicine
-
Nawata Hiroyuki
Third Department Of Internal Medicine Kinki University School Of Medicine
-
MATSUDA Mitsuhiro
Third Department of Internal Medicine, Kinki University School of Medicine
-
Kanamaru Akihisa
Division Of Hematology Nephrology And Rheumatology Department Of Internal Medicine Kinki University
-
Kanamaru Akihisa
Third Department Of Internal Medicine Kinki University School Of Medicine
-
Morita Yasuyoshi
Third Department Of Internal Medicine Kinki University School Of Medicine
-
Maeda Yasuhiro
Drug Safety Research Laboratory Taiho Pharmaceutical Co. Ltd.
-
Kikuchi Ako
Department Of Hematology/oncology Tokai University School Of Medicine
-
Kikuchi Ako
Tokai University
-
Miyazaki Yasushi
Department Of Hematology Tama Hokubu Medical Center
-
Kanamaru Akihisa
Division Of Hematology And Oncology Department Of Medicine Tokai University School Of Medicine
-
松田 光弘
熊本大 大学院自然科学研究科
-
Maeda Yasuhiro
Department Of Applied Physics Tokyo Institute Of Technology
-
Ando Kiyoshi
Department Of Hematology And Oncology Tokai University
-
Sumimoto Yoshiyasu
Third Department Of Internal Medicine Kinki University School Of Medicine
関連論文
- Randomized trial of response-oriented individualized versus fixed-schedule induction chemotherapy with idarubicin and cytarabine in adult acute myeloid leukemia : the JALSG AML95 study
- Comparative analysis of remission induction therapy for high-risk MDS and AML progressed from MDS in the MDS200 study of Japan Adult Leukemia Study Group
- Persistence of derivative chromosome 22 after achieving a major molecular response in chronic myeloid leukemia with a cryptic BCR-ABL1 fusion gene
- Phase I and II pharmacokinetic and pharmacodynamic study of the proteasome inhibitor bortezomib in Japanese patients with relapsed or refractory multiple myeloma
- Long-term efficacy of imatinib in a practical setting is correlated with imatinib trough concentration that is influenced by body size : a report by the Nagasaki CML Study Group
- Non-hematopoietic Mesenchymal Stem Cells can be Mobilized and Differentiate into Cardiomyocytes after Myocardial Infarction(Development and Differentiation 3 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Transplanted Bone Marrow Stem Cells Move to the Infarcted Myocardium and Regenerate the Heart
- Phase 1/2 clinical study of dasatinib in Japanese patients with chronic myeloid leukemia or Philadelphia chromosome-positive acute lymphoblastic leukemia
- Comparison of leukopenia between cytarabine and behenoyl cytarabine in JALSG AML-89 consolidation therapy
- No beneficial effect from addition of etoposide to daunorubicin,cytarabine,and 6-mercaptopurine in individualized induction therapy of adult acute myeloid leukemia:the JALSG-AML92 study
- Imatinib Provides Durable Molecular and Cytogenetic Responses in a Practical Setting for Both Newly Diagnosed and Previously Treated Chronic Myelogenous Leukemia : A Study in Nagasaki Prefecture, Japan
- Phase II study of a salvage regimen using cyclophosphamide, high-dose cytarabine, dexamethasone, etoposide, and rituximab in patients with relapsed or refractory B-cell non-Hodgkin's lymphoma
- Effects of Eicosapentaenoic Acid on Endothelial Cell-Derived Microparticles, Angiopoietins and Adiponectin in Patients with Type 2 Diabetes
- Chromosomal imbalances in adult T-cell leukemia revealed by comparative genomic hybridization: gains at 14q32 and 2p16-22 in cell lines
- A phase II, open-label, sequential-cohort, dose-escalation study of romiplostim in Japanese patients with chronic immune thrombocytopenic purpura
- Prognostic Significance of FLT3 Internal Tandem Duplication and NPM1 Mutations in Acute Myeloid Leukemia in an Unselected Patient Population
- Prospective isolation of regenerated cardiomyocytes from the mixture of mesenchymal stem cells
- Azole resistant Candida albicans in a leukemic patient : a case report
- Combination Chemotherapy With Risk Factor-Adjusted Dose Attenuation for High-Risk Myelodysplastic Syndrome and Resulting Leukemia in the Multicenter Study of the Japan Adult Leukemia Study Group (JALSG): Results of an Interim Analysis
- Clinical features of adult acute leukemia with 11q23 abnormalities in Japan : a co-operative multicenter study
- Broadly Tunable UV-Blue Picosecond Pulsed Laser and Its Application for Biological Imaging
- Mechanisms of retinoid resistance in a myeloid cell line, YM711,transfected with PML/RARα cDNA : possible involvement of redox potential
- Deformation structure in rapidly solidified Mg_97Zn_1Y_2 alloy with long period stacking order phase
- Dislocation Structure in Rapidly Solidified Mg_Zn_1Y_2 Alloy with Long Period Stacking Order Phase
- Production of High Strength Mg_ Zn_1 Y_2 Alloy by Using Mechanically Alloyed MgH_2 Powder
- Changes in Microbial Flora in Neutropenic Patients With Hematological Disorders After the Hanshin-Awaji Earthquake
- Myelodysplastic syndrome with B cell clonality in a patient five years after renal transplantation
- Recombinant human erythropoietin for late-onset anemia after allogeneic bone marrow transplantation
- Correlation between immunological abnormalities and prognosis in myelodysplastic syndrome patients
- Detection of donor-type alveolar macrophages in transplantation with highly purified CD34^+ bone marrow cells
- Myelodysplastic changes in three cases within 100 days after allogeneic bone marrow transplantation
- CORRELATION BETWEEN SEVERITY OF PARKINSONISM AND DOPAMINE NEURONAL DAMAGE IN MPTP-TREATED MARMOSETS
- Ex vivo evidence for asymmetric tyrosine phosphorylation of ZAP-70 on double-positive thymocytes in the positive selection process
- Extramedullary Blast Crisis Derived from 2 Different Clones in the Central Nervous System and Neck during Complete Cytogenetic Remission of Chronic Myelogenous Leukemia Treated with Imatinib Mesylate
- Follicular Lymphoma in Osaka, Japan : Histological Features and Chronological Change
- A Case of Hypoplastic Chronic Myelogenous Leukemia Initiating With Pancytopenia
- 953 パルス通電加圧焼結における焼結低合金鋼の組織制御
- MIM プロセスにおけるW-Cu系放熱部材の開発
- Superhigh Strength Metal Injection Molded Low Alloy Steels by In-Process Microstructural Control
- ヘテロ組織インプロセス制御による高機能動力伝達部品のネット成形 (ミニ特集「低環境負荷社会へのプロセス革新」)
- 薄肉・中空品のMIMインプロセス接合
- 627 高密度化と組織制御による焼結低合金鋼の超強靭化(ワークショップ 粉末成形・焼結の最前線)
- 焼結Fe-Ni合金鋼の高強度化
- 合金元素偏析を利用した焼結低合金鋼の超強靱化
- 合金元素偏析を利用した焼結低合金鋼の超強靭化
- 合金元素偏析を利用した焼結低合金鋼の超強靭化
- K-1011 MIMインプロセス接合(S12-1 粉末成形とその評価(1))(S12 粉末成形とその評価)
- 高密度化と組織制御による焼結低合金鋼の高性能化
- 焼結低合金鋼の高性能化に向けたメゾヘテロ組織制御(第2報)
- 1615 金属粉末射出成形法による焼結 Ni 合金鋼の衝撃破壊特性
- 焼結低合金鋼の高性能化に向けたメゾヘテロ組織制御
- 3 Lesson from GFP^+ bone marrow transplanted mice : Regeneration of infarcted myocardium by cytokine administration(Symposium 8 (SY8) (M) : Regeneration Medicine of Cardiovascular Diseases)(Special Program)
- 3-Dimensional Coronary Angiography in Mice Using Confocal : Laser Scanning Microscopy
- Ex Vivo Expansion of Human Cord Blood Stem Cells and Its Application to Gene Therapy
- A simple and efficient purification of transduced cells by using green fluorescent protein gene as a selection marker
- Possible involvement of the TGF-β1 signal in the development of peritoneal sclerosis as one of the major complications in continuous ambulatory peritoneal dialysis
- Potassium oxonate modulation of 5-fluororacil-induced myelotoxicity in colony forming assay of hematopoietic precursor cells.
- IMMUNOTOXIC EFFECT OF LOW DOSE CISPLATIN IN MICE
- Immunotoxicity Study of Cisplatin
- Comparative effects of three nitrosourea derivatives on cell cycle kinetics
- 13-cis Retinoic Acid Inhibits Growth of Adult T Cell Leukemia Cells and Causes Apoptosis; Possible New Indication for Retinoid Therapy
- Prognostic significance of bone marrow lymphocyte counts in the nadir phase of acute myelocytic leukemia
- Conception of Immunotherapy for Residual Leukemia Cells
- Serum KL-6 Levels in Patients With Pulmonary Complications After Allogeneic Bone Marrow Transplantation
- Ikk-i, a novel Lipopolysaccharide-inducible kinase that is realted to lκB Kinases
- Concurrent Murine Cytomegalovirus and Klebsiella pneumoniae Infections in Germfree Mice
- Immunological Responses in Germfree Mice Infected with Murine Cytomegalovirus
- Phase II study of oral fludarabine in combination with rituximab for relapsed indolent B-cell non-Hodgkin lymphoma
- Adult T-cell leukemia after immunosuppressive therapy for systemic lupus erythematosus
- Eosinophilic Colitis in a Patient with Acute Myeloid Leukemia after Allogeneic Bone Marrow Transplantation
- Human CD34^- Hematopoietic Stem Cells: Basic Features and Clinical Relevance
- The genomic structure and chromosomal localization of the mouse STAT3 gene
- Retinoid induces growth inhibition of adult T-cell leukemia cells
- Wavelength Stabilization of Dual-Wavelength Oscillation in Electronically Tuned Ti:Al2O3 Laser
- Characteristics of CD5-Positive Splenic Marginal Zone Lymphoma with Leukemic Manifestation ; Clinical, Flow Cytometry, and Histopathological Findings of 11 Cases
- Phase I study of the oral mammalian target of rapamycin inhibitor everolimus (RAD001) in Japanese patients with relapsed or refractory non-Hodgkin lymphoma
- Multicenter phase II study of bendamustine for relapsed or refractory indolent B-cell non-Hodgkin lymphoma and mantle cell lymphoma
- Phase I and pharmacokinetic study of bendamustine hydrochloride in relapsed or refractory indolent B-cell non-Hodgkin lymphoma and mantle cell lymphoma
- Clinical efficacy and safety of biapenem for febrile neutropenia in patients with underlying hematopoietic diseases : a multi-institutional study
- G0400-1-2 摩擦撹拌インクリメンタルフォーミング法によるA5052板の成形([G0400-1]機械材料・材料加工部門一般(1):金属材料と加工)
- EFFECTS OF CHRONIC ADMINISTRATION OF KANAMYCIN ON CONDITIONED SUPPRESSION TO AUDITORY STIMULUS IN RATS
- Crystallography and Morphology of Antiphase Boundary-Like Structure Induced by Martensitic Transformation in Ti-Pd Shape Memory Alloy
- Multi-Wavelength Spectroscopic Application Using Rapid and Random Wavelength-Tuned Mid-Infrared Light Source
- Safety and efficacy of the terminal complement inhibitor eculizumab in Japanese patients with paroxysmal nocturnal hemoglobinuria : the AEGIS Clinical Trial
- Lack of non-hematological cross intolerance of dasatinib to imatinib in imatinib-intolerant patients with Philadelphia chromosome positive chronic myeloid leukemia or acute lymphatic leukemia : a retrospective safety analysis
- Nilotinib as frontline therapy for patients with newly diagnosed Ph+ chronic myeloid leukemia in chronic phase : results from the Japanese subgroup of ENESTnd
- Romiplostim for the treatment of chronic immune thrombocytopenia in adult Japanese patients : a double-blind, randomized Phase III clinical trial
- Electronic Wavelength Tuning of Tunable Laser with Acousto-Optic Tunable Filter
- A New Type of Polydispersive Spin Relaxation in Mn-Zn Ferrite near T_c
- Feasibility and pharmacokinetic study of bendamustine hydrochloride in combination with rituximab in relapsed or refractory aggressive B cell non-Hodgkin's lymphoma
- Phase I and II study of azacitidine in Japanese patients with myelodysplastic syndromes
- Cytokine profiles in relapsed multiple myeloma patients undergoing febrile reactions to lenalidomide
- Transmission Electron Microscopy of Twins in 10M Martensite in Ni-Mn-Ga Ferromagnetic Shape Memory Alloy
- An open-label extension study evaluating the safety and efficacy of romiplostim for up to 3.5 years in thrombocytopenic Japanese patients with immune thrombocytopenic purpura (ITP)
- Temporal Changes in Erectile Function and Endothelium-Dependent Relaxing Response of Corpus Cavernosal Smooth Muscle After Ischemia by Ligation of Bilateral Internal Iliac Arteries in the Rabbit
- Effects of Psychoactive Drugs on Short-Term Memory in Rats and Rhesus Monkeys.
- G040053 摩擦撹拌インクリメンタルフォーミング法によるA2017板の成形([G04005]機械材料・材料加工部門一般セッション(5))