ガスクロマトグラフィーにおける脂肪酸定量法のプログラム化について

概要

論文の詳細を見る
A new type of gas-chromatography instrument with microcomputer (FID-663-50,Hitachi Co. Ltd.) was developted. We investigated the determination program for fatty acid methyl-esters and the obtained results were as follows; 1. The program is composed of analysis and determination programs. The former is composed of temperature sets, cut time, slope sense, sampling period, base line correction and minimun peak. The later is composed of retention time, factor coefficient, appointment of determination method. From these calculation processes, absolute value of each peak is calculated and printed out. 2. Temperature program should be set as to be obtained enough seperation on the gas-chromatography patterns. However, this program is not absolute and changes with packing condition of column and carrier gas flow. Our temperature program is composed of 120℃ of initial temperature, 7℃/min. of increasing rate of temperature and 230℃ of final temperature, and 17 kind of fatty acid methylesters were seperated enough under this temperature program. 3. Reproducibility of retention time of each fatty acid methylester by using the programed FID-663-50 is superior. Most fatty acid methylesters obtained from frogs, rats and human beings were analyzed enough in 40 min. 4. In the FID-663-50 instrument, base line correction is done by N-θ method. We set them as follows; N=1,θ=100μV/min. J=30 min. 5. Relationships among number of carbons, retention times, and factor coefficients were exponential. From these relationships, we could estimate unkown fatty acid methylesters by the retention times and the amounts by estimated factor coeffients and area values. Generally, unsaturated fatty acid methylesters have longer retention times and bigger factor coefficients than the fatty acid methylesters with same number of carbons.
東海大学の論文

ガスクロマトグラフィーにおける脂肪酸定量法のプログラム化について

スポンサーリンク

概要

著者

関連論文

スポンサーリンク