加令からみたラット副睾丸脂肪の脂肪分解能

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Lipolytic activity of adipose tissue with age has been widely investigated by many authors. There are differences on the conception of intrinsic Lipolytic activity of a cell changed with aging. Since lipolytic activity has been expressed by amount of FFA release per wet weight, per triglyceride or per protein, increase of triglyceride or wet weight seems to be a main factor to affect on it. It is of interest, therefore, to investigate the intrinsic Lipolytic activity of adipose tissue with aging from the relationship among FFA release, wet weight, protein an d triglyceride content in adipose tissue. In the present paper, we investigated effect of aging on lipolysis by using epididymal adipose tissue in 29, 35, 43, 57,105 and 350 days rats. Rats were anesthetized by intraperitoneal injection of pentobarbital sodium (4mg/100g body weight) and after that epididymal adipose tissue were removed and washed out the blood by 25℃ Krebs-Ringer solution. The fat pads were placed in glass bottles containing Krebs-Ringer bicarbonate buffer solution (pH 7.2-7.4), bovine serum albumin (4% w/v) and epinephrine (3.9μM/litre). Incubations were carried out in a shaking water bath for 120 min at 37℃ under an atmosphere of 95% O_2-5% CO_2. Rate of lipolysis were determined by assaying the production of free fatty acids. Free fatty acids were titrated by Dole's method. Triglyceride and protein content in fat pads were assayd by Stern and Shapiro's method and Lowry's method, respectively.
日本体力医学会の論文
1976-12-01

加令からみたラット副睾丸脂肪の脂肪分解能

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