<研究論文>長距離走者の赤血球膜脂質の変化

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This study was designed to clarify the influences of endurance training on membrane lipids of red blood cells (RBC) by using the ghost cells. The subjects were 21 young men, control group (n=11,18.5±3.5 years) and the long distance group (n=10,18.8±0.8 years). After removing the plasma and the white blood cells by water jet aspirator the RBC was diluted with 5mM phosphate butter and the white membrane of RBC was obtained by centrifugation at 10,000 rpm. Lipids extraction from the membrane was conducted by Folch Method and lipids separation and fatty acid determination were carried out by thinlayer and gas-chromatography. The following results were obtained. 1. There was not any differnce in the L/P ratio and cholesterol contents of the RBC membrane between control and long distance groups. 2. There was not difference in the phosphatidylcholine (PC) contents of the RBC membrane between control and long distance groups. 3. However, in the phoshpatidylethanolamine (PE) and sphyngomyerine (SP) significant decrease and increase occurred respectively (p < 0.05). 4. In these phospholipids, remarkable changes in fatty acid composition were noted; in particular, a decrease of arachidonic acids (C_<20 : 4>) and increase of nervonic acids (C_<24 : 1>) were recognized in both PE and PC. There results show that in the membrane lipids of RBC of long distance runners a significant decrease of PE and increase of SP occurred with changes in fatty acid composition, and we confirmed that endurance training clearly influences membrane lipids of the RBC.
東海大学の論文

<研究論文>長距離走者の赤血球膜脂質の変化

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