<研究論文>長距離選手のplasma particle imageによるmetabolic fieldの検討

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There are many particles in plasma such as lipoproteins, albumin, globulin, fragments of cell membrane and leaked enzymes from the cells. Flow cytometry can detect these sizes and functional structures and make a scatter graph using the forward scatter (FS) and photo multiplier tube PMT1; a plasma particle image (PPI). Changes in metabolic function induced by exercise or prolonged training directly influence the availability of PPI. To our kowledge there have been no report on the availability of PPI and the purpose of this study was to investigate the characteristics of PPI in student distance runners and the availability of PPI. The subjects were student distance runners (n=45), control subject (n=12), and a patient with myocardial infarction (n=1). Blood collection was performed in the early morning and the plasma was obtained by centrifugation at 3000rpm for 20min. The diluted plasma was used for flow cytometry. The stability of the PPI was clarified by plasma incubation (temperature, pH). The PPI of student distance runners contained many fragments of cell membranes such as WBC, platelet and DNA. The large FS fraction sorted by flow cytometry contained more TG and protein than the small FS fraction. In particular, different molecular proteins were found in the large FS fraction. The PPIs were roughly classified into 3 typical scatter graphs; small and round scatter graph with small FS and PMT1,swords type scatter graph with large FS and PMT1,and many swords type scatter graph with middle FS and PMT1. The PPIs of student distance runners were small and round type with small FS and PMT1 and there was a significance difference in FS, PMT1 and the number of the particles between student distance runners and the control students (p<0.0001). Jogging exercise caused changes in PPI with number of plasma particles FS and PMT1. Our findings showed the availability of PPI as a metabolic field and the characteristics of student distance runners.
東海大学の論文

<研究論文>長距離選手のplasma particle imageによるmetabolic fieldの検討

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