<原著>簡易カラー画像処理システムの開発と蛍光顕微鏡画像処理への応用

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A simplified and convenient color image processing system has been developed with a popular 16-bit microcomputer which could analyze the function and structure of various biological phenomena. This system is composed of a CPU with memories, a high-speed color image data acquisition board with 256×256 pixels, and input/output devices. The program handling the files and menues on the display are described with BASIC. The subroutines for data transfer, arithmetic and logical operations on the pixels are described with the machine codes for faster execution under MS-DOS. The color images of microscopic preparations with various stains were analyzed to calculate area, center of gravity, and distributions of each pixel as well as R, G and B components. The weakly illuminating fluorescent signals may be detected by a simple wide-band video amplifier. For prevention of UV-ray damage to the preparation, an electromagnetic shutter controlled by a timing circuit was inserted in the optical path between light source and microscope. A new program has been also developed for processing of faint images with a software, and R, G and B intensities of a pixel ; the enhancement of image signals may be obtained by averaging or integrating and then the noise components be reduced. An immunofluorescent image of bipolar cells with monoclonal antibody and fluorescent images such as dopaminergic neurons in the carp retina, cultured neuroblastoma cells with intracellular injection of Procion Yellow are processed to clarify the cell boundaries by amplifying the image signal of 3 to 4 times and adding image data over 10 times to the average. Experimentally processed images will help us to extract cell shape characteristics from the background and to measure the size of various neuronal cells.
近畿大学の論文
1989-06-25

<原著>簡易カラー画像処理システムの開発と蛍光顕微鏡画像処理への応用

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