<原著>抗ヒト・サイログロブリン・モノクロナル抗体の特異性についての検討

概要

論文の詳細を見る
Since Torrigiani et al. disclosed that the thyroglobulin (Tg) concentration in blood, which was found elevated in a case of thyroid carcinoma, was reduced down to the normal level in consequence of surgical intervention, the relationship between thyroid carcinoma and the blood level of Tg became a focus of debate as a tumor marker. In an attempt to study the immunological specificity of Tg in the papillary carcinoma of thyroid we have prepared anti human-Tg-monoclonal antibodies (mono-Ab). Before being able to apply these antibodies to the immunohistochemical studies we have investigated the specificity of 16 different mono-Ab's for Tg. To determine whether each antibodies can be identified as monoclonal, isoelectrophoresis of the mono-Ab's, determination of the IgG subclass and electrophoresis with cellulose acetate membrane were conducted. Further to evaluate the specificity of mono-Ab for human Tg cross verifications against diverse animal Tg's, the potency measurements through EIA, the inhibition test, and the stain of tissue through immunohistochemical technique were made in use. The results of these tests revealed that all the 16 kinds of mono-Ab's were identified as monoclonal and that they were provided with a specificity for human Tg.
近畿大学の論文
1985-09-25