Effect of nitric oxide on mouse clonal osteogenic cell, MC3T3-E1, proliferation in vitro
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概要
- 論文の詳細を見る
Nitric oxide (NO) is a very small lipophilic molecule which rapidly diffusesand reaches the cytoplasmic components, and results in the activation of diversebiological function. It has been already reported that cultured osteoblasts synthesizeNO in response to proinflammatory cytokines and lipopolysaccaride. In terms ofthe action of NO on bone metabolism, cytokine-induced NO by osteoblast inhibitsbone resorption through inducing the apoptosis of osteoblast progenitor cells andsuppressing the osteoblast activity. Also, NO synthase (NOS) inhibitor,NG-monomethyl-L-arginine is reported to induce a dose-dependent inhibitory effecton the proliferation of osteoblast-like cell lines MG63 and ROS 17/2.8, whichindicate that NO may stimulate cell proliferation. On the other hand,cytokine-induced NO is reported to reduce osteoblast activity significantly in highconcentration, as was evidenced by inhibition of DNA synthesis, cell proliferation,alkaline phosphatase activity, and osteocalcin production. Thus, the effect of NO onosteoblast activities is still controversial.In the present study, S-nitroso-N-acetyl-dl-penicillamine(SNAP), NO donorenhanced DNA synthesis of MC3T3-E1 in vitro. This activation seems to bemediated by NO directly because specific NO scavenger,2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-0xide (carboxy-PTIO) partially attenuated the osteoblast proliferation induced by SNAP. On theother hand, the guanylate cyclase inhibitor, LY83583, failed to abolish the effect ofSNAP on DNA synthesis of osteoblasts and 8-bromo cyclic guanosine3',5'-monophosphate (cGMP), substituting for the accumulation of intracellular cGMP in osteoblasts, did not enhance the incorporation of3H-thymidine (3H -TdR).It is, then, suggested that osteoblast proliferation might be enhanced by NOindependently apart from the activation of cytoplasmic guanylate cyclase andcGMP-dependent mechanisms.
- 神戸大学の論文
著者
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MIZUNO Kosaku
Department of Orthopaedic Surgery, Kobe University School of Medicine
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Saura Ryuichi
Department of Orthopaedic Surgery, Kobe University School of Medicine
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TAKADA Toru
Department of Orthopedic Surgery, Kobe University Graduate School of Medicine
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Takada Toru
Department Of Orthopaedic Surgery Kobe University Graduate School Of Medicine
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Takada Toru
Department Of Orthopaedic Surgery Kobe University School Of Medicine
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Mizuno Kosaku
Department Of Orthopaedic Surgery Kobe University School Of Medicine
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Saura Ryuichi
Department Of Orthopaedic Surgery Kobe University School Of Medicine
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Nakamaru Yuichi
Department of Orthopaedic Surgery, Kobe University School of Medicine
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Nakamaru Yuichi
Department Of Orthopaedic Surgery Kobe University School Of Medicine
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