Evaluation of hazardousness of refractory fibers by cell magnetometry

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概要

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• This study was carried out to assess the cytotoxicity of three kinds of refractory fibers (RFs), RF1, RF2, and RF3, by cell magnetometry, lactate dehydrogenase (LDH) assay and morphological observation by scanning electron microscopy, using a mouse-derived cultured cell line, RAW264.7. As an indicator for cell magnetometry, Fe3O4 was added to RAW264.7 cells. RF1, RF2 and RF3 were each added to an aliquot of this solution to make final concentrations of 250, 500 and 1,000 µg/ml in the experimental group. Phosphate buffered solution was added to make the control solution (n = 6). After culturing for 48 hr, the solution was magnetized from outside using a cell magnetometric apparatus, and the remnant magnetic field was measured for 20 min postmagnetization. In cell magnetometry, a significant delay of relaxation compared to that of the control was observed. In the LDH assay, LDH release into the culture medium was observed by addition of RFs. Furthermore, a quantity-dependent relationship was found between the quantity of RF added and the cytotoxicity in cell magnetometry and LDH assay. Morphological examination revealed incomplete phagocytosis of fibers and a decrease of microvilli in the experimental groups. These results suggest that RFs are cytotoxic to RAW264.7 cells, showing concentration-dependent cytotoxicity, and have a possible risk of cytotoxicity similar to that of asbestos. Further studies by pulmonary magnetometry are necessary to assess the hazardousness of RFs.

• 日本トキシコロジー学会の論文
• 2010-12-01

著者

• AIZAWA Yoshiharu

  Department of Preventive Medicine and Public Health, Kitasato University School of Medicine

• KUDO Yuichiro

  Department of Preventive Medicine and Public Health, Kitasato University School of Medicine

• Kudo Yasushi

  北里大学 医学部公衆衛生学

• Kudo Yasushi

  北里大学 医学部衛生学公衆衛学

• Kudo Yasushi

  北里大学 医学部公衆学･衛生学

• Aizawa Y

  Division Of Cardiology Niigata University Graduate School Of Medical And Dental Sciences

• Aizawa Yoshifusa

  新潟大学 医歯学総合研究科循環器学

• 相澤 好治

  北里大学 医学部衛生学公衆衛生学

• 相澤 好治

  北里大学 医学部 衛生学公衆衛生学教室

• 相澤 好治

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• Aizawa Yoshifusa

  新潟大学 医学部保健学科

• 相澤 好治

  北里大医・衛生公衆

• Aizawa Y

  First Department Of Internal Medicine Niigata University Graduate School Of Medical And Dental Scien

• 相澤 好治

  Dep. Of Preventive Medicine And Public Health Kitasato Univ. School Of Medicine

• 相澤 好治

  北里大学・医・衛生公衛

• Aizawa Yoshifusa

  Division Of Cardiology Niigata University Graduate School Of Medical & Dental Sciences

• Kudo Yasushi

  Dep. Of Preventive Medicine And Public Health Kitasato Univ. School Of Medicine

• Kudo Yuichiro

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• Kudo Yasushi

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• KOTANI Makoto

  Department of Electronics, Faculty of Engineering, Tokyo Denki University

• Aizawa Yoshifusa

  Division Of Cardiology Hemotology And Endocrinology Niigata University Graduate School Of Medical An

• Aizawa Yoshiharu

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• Aizawa Yosifusa

  First Department Of Internal Medicine Niigata University School Of Medicine

• Aizawa Yoshifusa

  Cardiovascular Medicine Niigata University Graduate School Of Medicine

• Kotani M

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• Aizawa Y

  新潟大学 内科

• Aizawa Yoshiharu

  Dep. Of Preventive Medicine And Public Health Kitasato Univ. School Of Medicine

• Aizawa Yoshifusa

  First Department Of Internal Medicine Niigata Universaity School Of Medicine

• Kotani Makoto

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• Aizawa Yoshifusa

  Division Of Endocrinology And Metabolism Department Of Homeostatic Regulation And Developments Niiga

• Aizawa Yoshifusa

  Division Of Cardiology Hemotology And Endocrinology Niigata University Graduate School Of Medical An

• Kudo Yuichiro

  Department Of Health Care Management Kitasato University School Of Medicine

• Aizawa Yoshifusa

  First Department Of Internal Medicine Niigata University Graduate School Of Medical And Dental Scien

• Aizawa Yoshiharu

  Department Of Occupational Health Graduate School Of Medical Sciences Kitasato University

• Kotani Makoto

  Department Of Electronic Engineering Tokyo Denki University

• AIZAWA YOSHIHARU

  Department of Internal Medicine, School of Medicine, Keio University

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