Evaluation of hazardousness of refractory fibers by cell magnetometry
スポンサーリンク
概要
- 論文の詳細を見る
This study was carried out to assess the cytotoxicity of three kinds of refractory fibers (RFs), RF1, RF2, and RF3, by cell magnetometry, lactate dehydrogenase (LDH) assay and morphological observation by scanning electron microscopy, using a mouse-derived cultured cell line, RAW264.7. As an indicator for cell magnetometry, Fe3O4 was added to RAW264.7 cells. RF1, RF2 and RF3 were each added to an aliquot of this solution to make final concentrations of 250, 500 and 1,000 µg/ml in the experimental group. Phosphate buffered solution was added to make the control solution (n = 6). After culturing for 48 hr, the solution was magnetized from outside using a cell magnetometric apparatus, and the remnant magnetic field was measured for 20 min postmagnetization. In cell magnetometry, a significant delay of relaxation compared to that of the control was observed. In the LDH assay, LDH release into the culture medium was observed by addition of RFs. Furthermore, a quantity-dependent relationship was found between the quantity of RF added and the cytotoxicity in cell magnetometry and LDH assay. Morphological examination revealed incomplete phagocytosis of fibers and a decrease of microvilli in the experimental groups. These results suggest that RFs are cytotoxic to RAW264.7 cells, showing concentration-dependent cytotoxicity, and have a possible risk of cytotoxicity similar to that of asbestos. Further studies by pulmonary magnetometry are necessary to assess the hazardousness of RFs.
- 日本トキシコロジー学会の論文
著者
-
AIZAWA Yoshiharu
Department of Preventive Medicine and Public Health, Kitasato University School of Medicine
-
KUDO Yuichiro
Department of Preventive Medicine and Public Health, Kitasato University School of Medicine
-
相澤 好治
Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine
-
KOTANI Makoto
Department of Electronics, Faculty of Engineering, Tokyo Denki University
関連論文
- Physician Job Satisfaction and Working Conditions in Japan
- PJ-474 The Effects of Erythropoietin-derivatives on Monocrotaline-induced Pulmonary Hypertension in Rats(PJ080,Pulmonary Circulation 1 (H),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- 保健所の精神保健活動における危機介入についての実態調査
- Effort-Reward Imbalance and Depression in Japanese Medical Residents
- A chronic myeloid leukemia patient with atypical karyotype and BCR-ABL e13a3 transcript caused by complex chromosome rearrangement
- トナー粒子における新たな生体影響調査と労働衛生管理について
- PJ-366 Phase I/II Clinical Study of Angiogenic Therapy by Ex-Vivo Expanded Erythroblast Transplantation (EVEETA) for the Patients with Critical Limb Ischemia(PJ062,Regeneration (Basic, Clinical) (M),Poster Session (Japanese),The 73rd Annual Scientific Mee
- シックハウス症候群に係わる医学的知見の整理
- Predictors of Japanese Workers' Motivation to Use the Results of Worksite Health Checkups in Their Daily Health Management
- SS5-5 シックハウス症候群と脳機能(IV,脳・心・神経系とアレルギー,生活環境習慣病としてのアレルギーを検証する,第59回日本アレルギー学会秋季学術大会)
- 日本公衆衛生学会における公衆衛生専門職制度の検討 (特集 公衆衛生の人づくり(2)専門性を支える公衆衛生人教育)
- 保健所管内における精神保健医療福祉資源についての全国調査 : 管内人口別、保健所型別分析
- The Effects of Dibutyltin (DBT) Dichloride on the Viability and the Productions of Tumor Necrosis Factor α and Interleukin-12 in Murine Macrophage Cell Line, J774.1
- 40.臨床医の慢性疲労に関連する要因の研究(ポスターセッション,第17回産業医・産業看護全国協議会,地方会・研究会記録)
- リフラクトリーセラミックファイバー2.0mgの気管内投与による肺内滞留性に関する研究
- 人工脳硬膜の安全性評価に関する研究(第2報) : 人工硬膜埋め込みラットの行動学試験を用いた神経毒性評価
- 環境医学の夢と現実
- 小・中学生の喫煙に関する意識と行動 : 地域における喫煙防止活動のために
- トナーの健康影響 (マテリアルスポット 毒性の強いナノ粒子と弱いナノ粒子,体内沈着までの挙動 ナノ粒子の吸引・毒性問題の最新の動き,対策手法のポイント)
- 助成論文 わが国の医療機関における産業保健活動の現状
- ホウ酸アルミニウムウイスカの細胞毒性および溶解性評価
- わが国における研修医のインシデント・アクシデントの現状
- Survey of Workers' Opinions Regarding Health Consultations by Occupational Physicians
- 関東地区の事業場における慢性疾患による仕事の生産性への影響
- Factors determining self-rated health : survey on evaluation of health status
- Classification of Patients Complaining of Sick House Syndrome and/or Multiple Chemical Sensitivity
- PJ-004 Clinical and ECG Characteristics as a Predictor of Recurrence of Ventricular Tachyarrhythmia (VTA) in Patients with Hypertrophic Cardiomyopathy (HCM)(PJ001,Arrhythmia, Others (Clinical/Pathophysiology) 1 (A),Poster Session (Japanese),The 73rd Annua
- 制約条件理論を用いた病院放射線科の業務効率化に向けた研究
- Lipocalin-2/Neutrophil Gelatinase-B Associated Lipocalin Is Strongly Induced in Hearts of Rats With Autoimmune Myocarditis and in Human Myocarditis
- Comparison of Antiarrhythmics Used in Patients With Paroxysmal Atrial Fibrillation : Subanalysis of J-RHYTHM Study
- Pharmacokinetic and pharmacodynamic analysis of cyclosporine A (CsA) to find the best single time point for the monitoring and adjusting of CsA dose using twice-daily 3-h intravenous infusions in allogeneic hematopoietic stem cell transplantation
- Telmisartan, an angiotensin-II receptor blocker ameliorates cardiac remodeling in rats with dilated cardiomyopathy
- PJ-692 Role of Faster Pacing and Magnesium (Mg) for the Treatment of Torsade de pointes (Tdp) Associated with Intravenous Amiodarone Administration(PJ116,Ventricular Arrhythmia (Clinical/Pathophysiology) (A),Poster Session (Japanese),The 73rd Annual Scien
- PJ-688 The Abnormality of QT/RR Relationship in Brugada Syndrome and Idiopathic Ventricular Fibrillation without Prominent J-wave(PJ116,Ventricular Arrhythmia (Clinical/Pathophysiology) (A),Poster Session (Japanese),The 73rd Annual Scientific Meeting of T
- PJ-555 Distribution of Defibrillation Threshold (DFT) in Patients Treated with Implantable Cardioverter-Defibrillators (ICD) : Incidence and Characteristics of High DFT Patients(PJ093,Ventricular Arrhythmia (Clinical/Diagnosis/Treatment) 2 (A),Poster Sess
- PJ-089 Characteristics of the Subsequent Recurrence of Ventricular Tachyarrhythmia in Patients under ICD Therapy, Concern of Driving(PJ015,ICD (M),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- PE-105 Exercise-induced QT Interval Prolongation and Its Relation to the Cardiac Events in Long QT Syndrome (LQTS) Carrying KCNQ1-Mutations(PE018,Arrhythmia, Others (Clinical/Pathophysiology) (A),Poster Session (English),The 73rd Annual Scientific Meeting
- 鼻部吸入曝露実験によるロックウールの有害性評価
- 中国東北部在宅高齢者に対する老老介護の実態とその問題
- 人工脳硬膜の安全性評価に関する研究 : 人工硬膜埋め込みラットの神経伝達物質を用いた神経毒性評価
- Dose-Response Effects of Bepridil in Patients With Persistent Atrial Fibrillation Monitored With Transtelephonic Electrocardiograms : A Multicenter, Randomized, Placebo-Controlled, Double-Blind Study (J-BAF Study)
- Sequential adenovirus infection of type 14 hemorrhagic cystitis and type 35 generalized infection after cord blood transplantation
- Serum Hepcidin-20 is Elevated during the Acute Phase of Myocardial Infarction
- PJ-525 MPO and MPO-ANCA in Coronary Artery Circulation(PJ088,ACS/AMI (Clinical/Pathophysiology) 2 (IHD),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- PE-167 Role of Mineralcorticoid Receptor Antagonist on Atrial Structural Remodeling in Hypertensive Rats(PE028,Heart Failure, Basic (M),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- 改良型N95マスク(DS2マスク)のフィットする割合の向上に関する研究
- OJ-146 Lipocalin-2 a New Biomarker of Inflammatory Heart Disease?(OJ25,Myocarditis, Basic/ Clinical (M),Oral Presentation (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- 磁界測定による化学物質の有害性評価
- 最近のシックハウス症候群研究の動向
- 平成18年の年頭にあたって
- 平成16年の年頭に当たって
- Associations of Excessive Sleepiness on Duty with Sleeping Hours and Number of Days of Overnight Work among Medical Residents in Japan
- Dibutyltin (DBT) Dichloride Inhibits Cytokine Productions in Murine Macrophage Cell Line, J774.1
- Magnetometric Evaluation for Cytotoxicity of Potassium Octatitanate Whisker on Alveolar Macrophages of Fischer 344 Rats
- In Vitro Toxicity of Gallium Arsenide in Alveolar Macrophages Evaluated by Magnetometry, Cytochemistry and Morphology
- Prognosis of Accidental Low Back Pain at Work
- Magnetometric Evaluation for the Effect of Chrysotile on Alveolar Macrophages
- Evaluation of hazardousness of refractory fibers by cell magnetometry
- Factors Influencing Male Workers' Dietary Habits
- Behavior of New Type of Rock Wool (HT Wool) in Lungs after Exposure by Nasal Inhalation in Rats
- Concentration in Blood and Organs of Dogs after High Dose 1,1,1-Trichloroethane Inhalation
- Adverse effects of colophony
- Magnetometric Evaluation of the Effects of Gallium Arsenide on the Clearance and Relaxation of Iron Particles
- Evaluation of hazardousness of refractory fibers by cell magnetometry
- Enhancing Work Motivation for Japanese Female Nurses in Small to Medium-Sized Private Hospitals by Analyzing Job Satisfaction
- Cytotoxicity Study of High Temperature Wool (HT Wool) by Cell Magnetometric Evaluation
- The differences of the effects of cigarette smoking according to race and ethnic background on serum cytokine levels among healthy men
- National Survey on Accidental Low Back Pain in Workplace
- An Epidemiological Study of Work-related Violence Experienced by Physicians who Graduated from a Medical School in Japan
- Development of Japanese Version of the Checklist Individual Strength Questionnaire in a Working Population
- Association between Intention to Stay on the Job and Job Satisfaction among Japanese Nurses in Small and Medium-sized Private Hospitals
- Behavior of Rock Wool in Rat Lungs after Exposure by Nasal Inhalation
- Factors Associated with Turnover Intention among Nurses in Small and Medium-Sized Medical Institutions
- The Cytotoxicity of Microglass Fibers on Alveolar Macrophages of Fischer 344 Rats Evaluated by Cell Magnetometry, Cytochemisry and Morphology
- Factors Associated with Satisfaction among Participants in a Periodical Worksite Health Check-Up in Japan
- The Pulmonary Hemodynamic Effects of 1,1,1-Trichloroethane Inhalation
- Odor Perception in Patients with Multiple Chemical Sensitivity
- Anxiety and Depressive States in Multiple Chemical Sensitivity
- Behavior of rock wool in lungs after exposure by nasal inhalation in rats
- Effects of Combination Therapy With Warfarin and Bucolome for Anticoagulation in Patients With Atrial Fibrillation
- A Novel Program to Accurately Quantify Infarction Volume by ^Tc MIBI SPECT, and Its Application for Re-Analyzing the Effect of Erythropoietin Administration in Patients With Acute Myocardial Infarction : A Randomized Controlled Pilot Trial of the EPO
- The alterations in cytokine mRNA expressions and productions by fluoride in a murine macrophage cell line evaluated by real-time PCR and ELISA
- Multiple Chemical Sensitivity and Idiopathic Environmental Intolerance(Part Two)
- Multiple Chemical Sensitivity and Idiopathic Environmental Intolerance(Part One)
- Antithrombotic Therapy in Atrial Fibrillation : Evaluation and Positioning of New Oral Anticoagulant Agents
- Building Medical Ethics Education to Improve Japanese Medical Students' Attitudes Toward Respecting Patients' Rights
- Clinical value of assessing the response to imatinib monitored by interphase FISH and RQ-PCR for BCR-ABL in peripheral blood for long-term survival of chronic phase CML patients : results of the Niigata CML-multi-institutional co-operative clinical study
- ST-T Abnormalities on ECG in Relation to Cardiovascular Risk Factors
- 復旧・復興作業における安全衛生対策 : 除染作業とこれからの災害に備えた石綿対策 (「東日本大震災」から1年 : 地震対策の見直しと復旧・復興作業時の安全衛生)
- Guidelines for Risks and Prevention of Sudden Cardiac Death (JCS 2010) : Digest Version
- Prevalence and Distribution of Sarcomeric Gene Mutations in Japanese Patients With Familial Hypertrophic Cardiomyopathy
- Association Between Lipid Profile and Risk of Atrial Fibrillation : Niigata Preventive Medicine Study
- 新型インフルエンザ発生時に企業に必要な感染対策に関する意思決定とそのための情報
- Work Motivation for Japanese Nursing Assistants in Small-to Medium-Sized Hospitals
- The Cholesterol Paradox in Atrial Fibrillation : Reply
- Bacterial Hazards of Sludge Brought Ashore by the Tsunami after the Great East Japan Earthquake of 2011
- Features of Japanese patients with myelodysplastic syndrome in an aging population of Sado Island
- Evaluation of Validity of the Urine Dipstick Test for Identification of Reduced Glomerular Filtration Rate in Japanese Male Workers Aged 40 Years and Over
- 災害時の産業保健活動
- O30-4 QEESIを用いたアレルギー疾患,シックハウス症候群,化学物質過敏症患者の症状比較(O30 アレルギーと環境要因,口演,第63回日本アレルギー学会秋季学術大会)