Magnetometric Evaluation for Cytotoxicity of Potassium Octatitanate Whisker on Alveolar Macrophages of Fischer 344 Rats

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概要

• 論文の詳細を見る

• Alveolar macrophages are thought to play a major role in the pathophysiology of lung diseases caused by exposure to various kinds of pathogens and particles. In the present study, the cytotoxic effect of potassium octatitanate whisker (PT) on macrophages was evaluated by means of magnetometry, lactate dehydrogenase (LDH) release measurement, apoptosis measurement and morphological observations. Alveolar macrophages obtained from Fischer rats (F344/N Slc) by bronchoalveolar lavage were incubated in vitro for 18 h with Fe_3O_4 as a magnetometric indicator, and PTs as test materials. In the control group and the group exposed to 10 ★g/ml of PT, rapid attenuation of the remanent magnetic field (RMF), so called "relaxation," was observed immediately after cessation of the external magnetic field. In comparison, a delay of relaxation was observed in alveolar macrophages exposed to 20 and 40 ★g/ml of PT. The decay constants, which are calculated from decreasing RMF for the first 2 mm, in the groups exposed to 20 and 40 ★g/ml of PT had significantly lower values than the control. LDH release induced by exposure to 20 and 40 ★g/ml of PT increased significantly in a concentration dependent manner in PT-exposed macrophages, whereas only negligible LDH release was observed in control groups. The level of PT affecting alveolar macrophages was at the same concentration, and in a dose-dependent manner among relaxation, decay constant and LDH measurement. A DNA ladder detection method and morphological observations detected no apoptosis in PT-exposed macrophages. Electron microscopic examination revealed vacuolar changes and cell membrane damage in PT-exposed macrophages, but no significant changes in control macrophages. The results of magnetometry, LDH release, apoptosis measurement and electron microscopic observations suggest concentration dependent cytotoxicity caused by exposure of alveolar macrophages to PT.

• 社団法人日本産業衛生学会の論文

著者

• AIZAWA Yoshiharu

  Department of Preventive Medicine and Public Health, Kitasato University School of Medicine

• OKADA Mitsushi

  Department of Preventive Medicine and Public Health, Kitasato University School of Medicine

• SATOH Toshihiko

  Kitasato Clinical Research Center, Kitasato University School of Medicine

• KUDO Yuichiro

  Department of Preventive Medicine and Public Health, Kitasato University School of Medicine

• SATOH Toshihiko

  Department of Preventive Medicine and Public Health, Kitasato University School of Medicine

• WATANABE Mitsuyasu

  Department of Preventive Medicine and Public Health, Kitasato University School of Medicine

• Kudo Yasushi

  北里大学 医学部衛生学公衆衛学

• Kudo Yasushi

  北里大学 医学部公衆学･衛生学

• Okada M

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• Okada Mitsushi

  北里大学 衛生公衆衛生

• Okada Mitsushi

  Dep. Of Preventive Medicine And Public Health Kitasato Univ. School Of Medicine

• Sato Toshihiko

  Kitasato Clinical Research Center Kitasato University School Of Medicine

• Kudo Yasushi

  Dep. Of Preventive Medicine And Public Health Kitasato Univ. School Of Medicine

• Kudo Yuichiro

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• Kudo Yasushi

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• SHIBATA Kaori

  Graduate School of Medical Sciences, Kitasato University

• NIITSUYA Masato

  Department of Preventive Medicine and Public Health, Kitasato University School of Medicine

• KOTANI Makoto

  Department of Electronics, Faculty of Engineering, Tokyo Denki University

• Aizawa Yoshiharu

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• Satoh Toshihiko

  Kitasato Clinical Research Center Kitasato University School Of Medicine

• Kotani M

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• Okada Mitsushi

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• Sakabe Kou

  Dep. Of Public Health And Molecular Toxicology Kitasato Univ. School Of Pharmaceutical Sciences

• Watanabe Mituyasu

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• Niitsuya Masato

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• Niitsuya Masato

  Department Of Internal Medicine And Pathology

• Kotani Makoto

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• Watanabe Mitsuyasu

  Dep. Of Preventive Medicine And Public Health Kitasato Univ. School Of Medicine

• Watanabe Mitsuyasu

  Department Of Preventive Medicine And Public Health Kitasato University School Of Medicine

• Satoh Toshihiko

  Department Of Hygiene And Public Health Tokyo Women's Medical College

• Kudo Yuichiro

  Department Of Health Care Management Kitasato University School Of Medicine

• Aizawa Yoshiharu

  Department Of Occupational Health Graduate School Of Medical Sciences Kitasato University

• Kotani Makoto

  Department Of Electronic Engineering Tokyo Denki University

• AIZAWA YOSHIHARU

  Department of Internal Medicine, School of Medicine, Keio University

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