クロロフィル蛍光画像を用いた Amphidinium sp. の藻体濃度および光合成電子伝達収率の評価

概要

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In this study, chlorophyll fluorescence imaging of the maximal yield in the dark was applied to estimation of cell concentration of Amphidinium sp. As the result, a linear relationship was observed between chlorophyll fluorescence intensity and cell concentration. It was revealed that the method enabled rapid measurement of cell concentration without effect of chlorophyll fluorescence induction. To investigate the effect of light qualities during the culture on photosynthetic activities of Amphidinium sp., changes in ΦPSII of the algae cultivated under red, blue and red+blue lights were compared. As the result, the ΦPSII of algae cultivated under red+blue light was higher than that under blue light. It was implied that the light quality affected the efficiency of the photosynthetic electron transport. Meanwhile, the ΦPSII of algae cultivated under red light was similar to that under red+blue light in spite of its low growth rate. It was implied that the heat dissipation of Amphidinium sp. cultivated under red light did not increase because their low chlorophyll concentration led to low light absorbance.
生態工学会の論文
2008-01-31