励起-蛍光マトリックスによる Amphidinium sp. の成育過程のモニタリング

概要

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In this study, the excitation-emission matrix (EEM) was used to select suitable wavelengths for monitoring growth process of Amphidinium sp. As the result, the chlorophyll fluorescence intensity at 680 nm excited by the 470 nm light (E470 F680) which was suitable for measuring cell concentration changed with reflection intensity of suspension in a culture flask. Meanwhile, the fluorescence intensity at 620 nm excited by the 400 nm light (E400 F620) increased with culture time even when cell concentration of Amphidinium sp. decreased. It was implied that the E400 F620 matter was an exudate of porphyrin-like compounds derived from photooxidized chlorophyll (Chl). The EEM spectroscoped both the excitation and emission wavelengths, therefore the EEM was an efficient method to detect the increase of the compounds because the excitation and fluorescence wavelengths of the compounds were neighbouring those of Chl a. Consequently, it was able to simultaneously monitor living and dead cell concentrations by measuring E470 F680 and E400 F620.
生態工学会の論文
2007-10-31