Cardiolipin Enrichment in Spore Membranes and Its Involvement in Germination of Bacillus subtilis Marburg
スポンサーリンク
概要
- 論文の詳細を見る
Examination of the lipid composition of spore membranes of Bacillus subtilis Marburg, extracted after treatment of spores with dithiothreitol/urea and NaOH followed by lysozyme digestion, revealed that the spore membranes had significantly higher cardiolipin (CL) content than the membranes of exponentially growing cells. Analysis of the membranes of coat-defective, cotE::cat and gerE::cat mutant spores, which are susceptible to lysozyme digestion without chemical treatment, confirmed that spore membranes contain a high level of CL. After addition of the germinants L-alanine or AGFK (a combination of asparagine, glucose, fructose, and KCl), the turbidity of wild type spore suspensions decreased to 50% within 30 min. Suspensions of spores with only trace amounts of CL, however, showed no decrease in turbidity when L-alanine was added and the initial decrease in turbidity with AGFK was slight (14% after 60 min). These results indicate that CL is involved in an early step of germination, related to the functioning of germinant receptors. This is the first conspicuous in vivo evidence that CL in bacterial membranes has a specific role, in which it cannot be replaced by other anionic phospholipids.
- 日本遺伝学会の論文
- 2006-04-25
著者
-
HARA Hiroshi
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University
-
Matsumoto Kouji
Department of Biochemistry and Molecular Biology, Saitama University
-
Watabe Kazuhito
Faculty Of Pharmaceutical Sciences Setsunan University
-
Watabe Kazuhito
Faculty Of Pharmaceutical Science Setsunan University
-
Matsumoto Kouji
Department Of Biochemistry And Molecular Biology Graduate School Of Science And Engineering Saitama
-
Takamatsu Hiromu
Faculty Of Pharmaceutical Sciences Setsunan University
-
KAWAI Fumitaka
Department of Biochemistry and Molecular Biology, Saitama University
-
Matsumoto Kouji
Department Of Biochemistry And Molecular Biology Saitama University
-
Hara Hiroshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tokyo Noko University
-
Kawai Fumitaka
Department Of Biochemistry And Molecular Biology Saitama University
-
Hara Hiroshi
Department Of Biochemistry And Molecular Biology Saitama University
-
HARA Hiroshi
Department fo Bioscience and Chemistry, Faculty of Agriculture
関連論文
- Effects of Germinated Barley Foodstuff in Preventing Diarrhea and Forming Normal Feces in Ceco-colectomized Rats
- DjlA negatively regulates the Rcs signal transduction system in Escherichia coli
- Long-Term Survival Following Treatment with Antineoplastons for Colon Cancer with Unresectable Multiple Liver Metastases : Report of a Case
- Modification of GerQ Reveals a Functional Relationship between Tgl and YabG in the Coat of Bacillus subtilis Spores
- RECQ5/QE DNA Helicase Interacts with Retrotransposon mdg3 gag, an HIV Nucleocapsid-Related Protein
- Guanidinated Casein Hydrolysate Stimulation of Cholecystokinin Release via Pancreatic Enzyme-and Cholinergic-independent Mechanisms in Rats
- Directed Mutagenesis, Ser-56 to Pro, of Bacillus subtilis Phosphatidylserine Synthase Drastically Lowers Enzymatic Activity and Relieves Amplification Toxicity in Escherichia coli
- TWO-DIMENSIONAL ECHOCARDIOGRAPHIC MEASUREMENT OF LEFT VENTRICULAR VOLUME : New Techniques, Echocardiography II : PROCEEDINGS OF THE 44th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Cushing syndrome associated with prostatic tumor adrenocorticotropic hormone (ACTH) expression after maximal androgen blockade therapy
- Quantitative Evaluation of the Gastrointestinal Absorption of Protein into the Blood and Lymph Circulation
- Inhibitory Effect of Plant Extracts on Production of Verotoxin by Enterohemorrhagic Escherichia coil O157 : H7
- Clinical evaluation of urolithiasis in Crohn's disease
- Measurement of the Rate of Whole Body Protein Synthesis by Intraperitoneal Injection of a Large Dose of Alanyltyrosine with [^C]Tyrosine(Food & Nutrition)
- Antibacterial Activity of Extracts Prepared from Tropical and Subtropical Plants on Methicillin-Resistant Staphylococcus aureus
- A Novel Antileukemic Tropoloisoquinoline Alkaloid, Pareirubrine, from Cissampelos pareira
- Growth Potencies and Effects on Digestive Functions of an Amino Acid and a Casein Diet in Rats of Three Strains
- Suppression of the Lethal Effect of Acidic-Phospholipid Deficiency in Escherichia coli by Bacillus subtilis Chromosomal Locus ypoP
- A Regulatory Mechanism for the Balanced Synthesis of Membrane Phospholipid Species in Escherichia coli
- Acidic-phospholipid Deficiency Represses the Flagellar Master Operon through a Novel Regulatory Region in Escherichia coli
- Flagellar Formation Depends on Membrane Acidic Phospholipids in Escherichia coli
- Duration of sexual intercourse related to satisfaction : survey of Japanese married couples
- Increased Intestinal Calcium Absorption from the Ingestion of a Phosphorylated Guar Gum Hydrolysate Independent of Cecal Fermentation in Rats
- Effect of a Phosphorylated Guar Gum Hydrolysate on Increased Calcium Solubilization and the Promotion of Calcium Absorption in Rats
- Spore Germinability of Bacillus coagulans and Acidophile Bacillus spp. Isolated from PET-bottled Tea and Juices
- Stimulative Effect of a Casein Hydrolysate on Exocrine Pancreatic Secretion That is Independent of Luminal Trypsin Inhibitory Activity in Rats
- Guanidino Group Is Involved in the Stimulation of Exocrine Pancreatic Secretion by Protamine in Normal and Chronic Bile-Pancreatic Juice-Diverted Rats
- Stimulation of Exocrine Pancreatic Secretion by Soybean Trypsin Inhibitor Does Not Depend on the Masking of Luminal Trypsin Activity in Rats That Have Bile-Pancreatic Juice Diverted into the Ileum
- Giant cystic basal cell carcinoma mimicking epidermal cyst
- EFFECT OF VORTEX CELL STRUCTURE ON BIFURCATION PROPERTIES IN A TAYLOR VORTEX FLOW SYSTEM
- TWO-DIMENSIONAL ECHOCARDIOGRAPHIC CHARACTERISTICS OF BICUSPID AORTIC VALVE : Echocardiography : FREE COMMUNICATIONS (Abstract) : 45 Annual Scientific Meeting, Japanese Circulation Society
- TWO-DIMENSIONAL ECHOCARDIOGRAPHIC MEASUREMENT OF RIGHT VENTRICULAR VOLUME : Echocardiography : FREE COMMUNICATIONS (Abstract) : 45 Annual Scientific Meeting, Japanese Circulation Society
- ACCURATE LEFT VENTRICULAR VOLUME DETERMINATION BY TWO DIMENSIONAL ECHOCARDIOGRAPHY : Echocardiography : FREE COMMUNICATIONS (Abstract) : 45 Annual Scientific Meeting, Japanese Circulation Society
- Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with membranes of reduced phosphatidylglycerol content
- Effect of Residual CO on Combustion-Type CO_2-Gas Dynamic Laser Performance
- A Novel Small Protein of Bacillus subtilis Involved in Spore Germination and Spore Coat Assembly
- Synthesis and Characterization of the Spore Proteins of Bacillus subtilis YdhD, YkuD, and YkvP, Which Carry a Motif Conserved among Cell Wall Binding Proteins^1
- IV F13 Effects of YM872,a selective AMPA receptor antagonist, in the rat kindling model of epilepsy
- Expression, Localization and Modification of YxeE Spore Coat Protein in Bacillus subtilis
- GC-MS Analysis of Organic Compounds in Rain Water (Regular Presentations) (Proceedings of the 9th Symposium on Environmental Pollutants and Toxicology)
- Changes in Messenger RNA of Pancreatic Enzymes and Intestinal Cholecystokinin after a 7-Day Bile-pancreatic Juice Diversion from the Proximal Small Intestine in Rats
- Impaired Pancreatic Acinar Sensitivity to Cholecystokinin But Not to Carbachol in Rats with Chronic Bile Pancreatic Juice Diversion
- Electroluminescence in Perylene-and-Tetracene-Doped Anthracene Films
- Electroluminescence in Perylene-Doped Anthracene Films : The Ambient Gas Effect in the Emission Process
- Effects of Difructose Anhydride III on Calcium Absorption in Small and Large Intestines of Rats
- STUDY ON THE ENVIRONMENTAL EVALUATION OF RIVER WATER USING BACTERIA AS BIOLOGICAL INDICATOR
- Renal Adaptation of Ammonia-producing Enzymes for NH_4Cl-induced Acidosis
- BLOOD CLEARANCE OF ^<99m>Tc-PHYTATE FOR EVALUATION OF HEPATIC DYSFUNCTION IN RATS
- Involvement of PlsX and the acyl-phosphate dependent sn-glycerol-3-phosphate acyltransferase PlsY in the initial stage of glycerolipid synthesis in Bacillus subtilis
- Reducing Sludge Production and the Domination of Comamonadaceae by Reducing the Oxygen Supply in the Wastewater Treatment Procedure of a Food-Processing Factory
- Gastric Acid-Independent Enhancement of Exocrine Pancreatic Secretion by Dietary Protein in Chronic Bile-Pancreatic Juice Diverted Rats
- Osmotic Pressure of the Chyme in the Gastrointestinal Tract of Rats Fed with a Toxic Dose of Amaranth (FD & C Red No. 2) and Dietary Fiber
- Cardiolipin Enrichment in Spore Membranes and Its Involvement in Germination of Bacillus subtilis Marburg
- Antimicrobial Effect of Chemical Preservatives on Enterohemorrhagic Escherichia coli O157 : H7
- Comparison of Standard Coronary Artery Bypass Grafting and Minimary Invasive Direct Coronary Artery Bypass Grafting Early and Midterm Result
- In Vivo Absorption of Calcium Carbonate and Magnesium Oxide from the Large Intestine in Rats
- A Novel Small Protein of Bacillus subtilis Involved in Spore Germination and Spore Coat Assembly
- Characterization of the Nuclear- and Plastid-Encoded secA-Homologous Genes in the Unicellular Red Alga Cyanidioschyzon merolae
- Visual Laser Ablation with Transurethral resection of the Prostate (Laser assisted TURP) for the Treatment of Benign Prostatic Hyperplasia
- The Bacillus subtilis essential gene dgkB is dispensable in mutants with defective lipoteichoic acid synthesis
- Substrate specificity of SpollGA, a signal-transducing aspartic protease in Bacilli
- Abnormal morphology of Bacillus subtilis ugtP mutant cells lacking glucolipids
- Expression and Localization of Two SecA Homologs in the Unicellular Red Alga Cyanidioschyzon merolae
- Discrimination of the Bacillus cereus Group Members by Pattern Analysis of Random Amplified Polymorphic DNA-PCR
- Coronary bypass surgery after thrombolytic therapy for total occlusion of the left main coronary artery.
- A case report of coronary revascularization for Kawasaki disease.
- Modified De Vega's Annuloplasty for Functional Tricuspid Regurgitation. Early and Late Results.:Early and Late Results
- Toxicological Study on Antineoplastons A-10 and AS2-1 in Cancer Patients.
- Biochemical and morphological changes in isolated rat liver mitochondria exposed to ultraviolet light.
- Photolysis of Ethylene at 1634 Å and 1849 Å
- Energized Vinyl Radical Formation in the Photolysis of Ethylene
- Inhibitory Effect of Antineoplaston A10 and AS2-1 on Human Hepatocellular Carcinoma.
- Synthesis and Characterization of the Spore Proteins of Bacillus subtilis YdhD, YkuD, and YkvP, Which Carry a Motif Conserved among Cell Wall Binding Proteins.