DjlA negatively regulates the Rcs signal transduction system in Escherichia coli
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概要
- 論文の詳細を見る
The Rcs signal transduction system of Escherichia coli regulating capsularpolysaccharide synthesis (cps) genes is activated by overexpression of the djlA gene encodinga cytoplasmic membrane-anchored DnaJ-like protein. However, by monitoring the expression of a cpsB′-lac fusion in pgsA- and mdoH-null mutants in which the Rcs system isactivated, we found that the Rcs activity was further increased by deletion of djlA anddecreased by low-level extrachromosomal expression of djlA. Furthermore, deletion of djlA ina wild-type strain led to small but significant increase of the basal-level activity of the Rcssystem. These results demonstrate that DjlA functions as a negative regulator of the Rcs system unless abnormally overproduced.
- 2006-02-25
著者
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HARA Hiroshi
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University
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Matsumoto Kouji
Department Of Biochemistry And Molecular Biology Graduate School Of Science And Engineering Saitama
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Matsumoto Kouji
The Graduate School Of Sci. And Engineering Saitama Univ.
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SHIBA Yasuhiro
Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University
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Hara Hiroshi
Department Of Biochemistry And Molecular Biology Graduate School Of Science And Engineering Saitama
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Shiba Yasuhiro
Department Of Biochemistry And Molecular Biology Faculty Of Science Saitama University
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Hara Hiroshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tokyo Noko University
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Hara Hiroshi
Department Of Biochemistry And Molecular Biology Faculty Of Science Saitama University
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HARA Hiroshi
Department fo Bioscience and Chemistry, Faculty of Agriculture
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