Effect of Bombyx mori Chitinase against Japanese Pine Sawyer (Monochamus alternatus) Adults as a Biopesticide
スポンサーリンク
概要
- 論文の詳細を見る
Bombyx mori chitinase (Bm-CHI), with a molecular mass of 75 kDa, was investigated on the possibility that it can serve as a biocontrol agent against the adult Japanese pine sawyer (JPS), Monochamus alternatus (Coleoptera: Cerambycidae). Oral ingestion of purified chitinase at concentrations of 3 μM (11.25 μg/50 μl) and 0.3 μM (1.125 μg/50 μl) caused high mortality in JPS, a significant decrease in bark consumption, and, only in high concentration, a slight reduction of body weight. Fluorescence assays indicated that peritrophic membrane (PM) chitin is degraded by the action of orally ingested Bm-CHI at 3 μM concentration only. Scanning electron micrographs clearly indicated that the beetles that ingested Bm-CHI of the same high concentration had their PM perforated and disrupted, but ultrastructural studies showed that the ingested chitinase did not affect the midgut epithelium. These findings open up the possibility of using insect chitinase as a biopesticidal enzyme. It should have agronomic potential for insect control.
- 社団法人 日本農芸化学会の論文
- 2006-01-23
著者
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田戸 裕之
山口県農林総合技術センター
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Kabir Khondkar
Department Of Biological Science Faculty Of Agriculture Yamaguchi University
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TANAKA SHUHEI
Department of Plant Pathology, Faculty of Agriculture, Yamaguchi University
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Yamanaka Akira
Department of Thoracic Surgery, Chest Diseases Research Institute, Kyoto University
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Endo K
Department Of Physics Biology And Informatics Faculty Of Science Yamaguchi University
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Koga D
Department Of Biological Science Faculty Of Agriculture Yamaguchi University
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Koga Daizo
Department Of Biological Science Faculty Of Agriculture Yamaguchi University
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Yamanaka A
Laboratory Of Environmental Biology Graduate School Of Science And Engineering Yamaguchi University:
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Yamanaka Akira
Yamaguchi Univ. Yamaguchi Jpn
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Endo Katsuhiko
Department of Physics, Biology and lnformatics, Faculty of Science, Yamaguchi University
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Endo Katsuhiko
Department Of Physics Biology And Informatics Faculty Of Science Ymanaguchi University
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SUGIMOTO Hiroyuki
Yamaguchi Prefectural Forestry Guidance Institute
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TADO Hiroyuki
Yamaguchi Prefectural Forestry Guidance Institute
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Tado Hiroyuki
Management Of Technique Research Lab Yamaguchi Prefecture Agriculture And Forestry General Engineeri
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Yamanaka Akira
Institute Of Biological Science Faculty Of Science Yamaguchi University
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Yamanaka Akira
Department Of Physics Biology And Informatics Faculty Of Science Yamaguchi University
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Tanaka Shuhei
Department Of Biological And Environmental Sciences Faculty Of Agriculture Yamaguchi University
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Tanaka Shuhei
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University
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KOGA Daizo
Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University
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