Purification and Characterization of Biliverdin-binding Protein from Larval Hemolymph of the Swallowtail Butterfly, Papilio xuthus L.
スポンサーリンク
概要
- 論文の詳細を見る
The biliverdin-binding protein from the larval hemolymph of the swallowtail butterfly, Papilio xuthus L., was purified and characterized. The crude biliverdin-binding protein, obtained by ammonium sulfate fractionation, was purified in two steps, the first one by gel filtration chromatography and the second one by ionexchange chromatography. The molecular mass of the purified protein was analyzed by SDS-polyacrylamide gel electrophoresis and estimated to be 21 kDa. The Namino terminal sequence of P. xuthus biliverdin-binding protein analyzed up to the 19th residue showed that 42% of the amino acid sequence are sequence similarity to the bilin-binding protein from Pieris brassicae. These results suggest that the P. xuthus biliverdin-binding protein belongs to the insecticyanin-type.
- 社団法人日本農芸化学会の論文
- 2000-09-23
著者
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Sato Toshiyuki
Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine
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Sato Toshiyuki
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Yamanaka Akira
Department of Thoracic Surgery, Chest Diseases Research Institute, Kyoto University
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Endo K
Department Of Physics Biology And Informatics Faculty Of Science Yamaguchi University
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Koga D
Department Of Biological Science Faculty Of Agriculture Yamaguchi University
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Koga Daizo
Department Of Biological Science Faculty Of Agriculture Yamaguchi University
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Yamanaka A
Laboratory Of Environmental Biology Graduate School Of Science And Engineering Yamaguchi University:
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Yamanaka Akira
Yamaguchi Univ. Yamaguchi Jpn
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Ochiai M
Biochemistry Laboratory Institute Of Low Temperature Science Hokkaido University
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Endo Katsuhiko
Department of Physics, Biology and lnformatics, Faculty of Science, Yamaguchi University
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Endo Katsuhiko
Department Of Physics Biology And Informatics Faculty Of Science Ymanaguchi University
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ITO Takamasa
Department of Physics, Biology and Informatics, Faculty of Science, Yamaguchi University
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OCHIAI Masanori
Biochemical Laboratory, The Institute of Low Temperature Science, Hokkaido University
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Ito Takamasa
Development Of Physics Biology And Informatics Faculty Of Science Yamaguchi University
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Yamanaka Akira
Institute Of Biological Science Faculty Of Science Yamaguchi University
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Yamanaka Akira
Department Of Physics Biology And Informatics Faculty Of Science Yamaguchi University
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Sato Toshiyuki
Department Of Biology Faculty Of Science Shinshu University
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KOGA Daizo
Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University
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