Regeneration of transformed Ophiorrhiza pumila plants producing camptothecin
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概要
- 論文の詳細を見る
Camptothecin derivatives are clinically used as anti-tumor alkaloids that are currently obtained by extraction from intact plants. Seeking for the alternative sources for commercial production and for fundamental study, cell and tissue cultures have been investigated. In the present study, we developed a method for regeneration of Ophiorrhiza pumila plant from hairy roots transformed with Agrobacterium rhizogenes. The regeneration frequency was over 83%. Integration of a rol B gene from T-DNA of A. rhizogenes was confirmed by polymerase chain reaction in both of the hairy roots and the regenerated plants. The transformed plants accumulated camptothecin in amounts of 66–111% compared with that in the wild-type plants.
- 日本植物細胞分子生物学会の論文
- 2004-12-01
著者
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YAMAZAKI Mami
Department of Oral Pathology, School of Dentistry, Health Sciences University of Hokkaido
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SAITO KAZUKI
Department of Pharmacology, School of Medicine, Keio University
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WATASE Ikumi
Graduate School of Pharmaceutical Sciences, Chiba University
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Yamazaki Mami
Department Of Molecular Biology And Biotechnology Graduate School Of Pharmaceutical Sciences Chiba U
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SUDO Hiroshi
Department of Internal Medicine, Tokai University School of Medicine
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WATASE Ikumi
Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba
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Sudo Hiroshi
Department Of Internal Medicine Tokai University School Of Medicine
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Watase Ikumi
Graduate School Of Pharmaceutical Sciences Chiba University
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Yokoya M
Chiba Univ. Chiba Jpn
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Saito Kazuki
Department Of Molecular Biology And Biotechnology Graduate School Of Pharmaceutical Sciences Chiba U
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Sudo H
Department Of Molecular Biology And Biotechnology Graduate School Of Pharmaceutical Sciences Chiba U
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Saito Kazuki
Department Of Chemistry School Of Science Tokai University
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Yamazaki Mami
Department Of Molecular Biology And Biotechnology Graduate School Of Pharmaceutical Sciences Chiba U
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Saito Kazuki
Department Of Molecular Biology And Biotechnology Graduate School Of Pharmaceutical Sciences Chiba U
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Saito Kazuki
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo
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