Mass Spectrometric Studies of Peptides from Cyanobacteria under FAB MS/MS Conditions
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概要
- 論文の詳細を見る
For the elucidation of the biosynthetic relationship between nontoxic peptides and hepatotoxic peptides produced together with hepatotoxic peptides, we applied the high-energy collision FAB MS/MS method to the confirmation and the determination of the peptide structure isolated from cyanobacteria. The structures of the cyanobacterial peptides including the cyclic peptides were definitely elucidated by complementary use of the NMR and MS/MS methods. Furthermore, the MS/MS method is very effective for the structural determination of closely related peptides derived from the difference of a part of the constituent amino acids, and their structures were determined by MS/MS experiments and amino acid analysis without NMR techniques. Additionally, the charge-remote fragmentation was observed in the cases of peptides containing Arg and the sulfate group which facilitated the interpretation of the resulting product ion spectrum. From the present study, the MS/MS method was found to be practicable for the confirmation and determination of the structures of cyanobacterial peptides.
- 日本質量分析学会の論文
- 2000-02-01
著者
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HARADA Ken-ichi
Faculty of Pharmacy, Meijo University
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FUJII KIYONAGA
Faculty of Pharmacy, Meijo University
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MAYUMI Tsuyoshi
Faculty of Pharmacy, Meijo University
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HIRAYAMA Kazuo
Central Research Laboratories, Ajinomoto Co., Inc.
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Sivonen Kaarina
Department Of Applied Chemistry And Microbiology
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NOGUCHI Kazuyoshi
Central Research Laboratories, Ajinomoto Co., Inc.
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Harada Ken-ichi
Faculty Of Pharmacy Meijo University
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Akashi Satoko
Central Research Laboratories Ajinomoto Co. Inc.
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Fujii Kiyonaga
Faculty Of Pharmacy Meijo University
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Hirayama K
Central Research Laboratories Ajinomoto Co. Inc.
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Hirayama Kazuo
Central Research Laboratories Ajinomoto Co. Inc.
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Mayumi Tsuyoshi
Faculty Of Pharmacy Meijo University
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KASHIWAGI Tatsuki
Central Research Laboratories, Ajinomoto Co., Inc.
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Kashiwagi Tatsuki
Central Research Laboratories Ajinomoto Co. Inc.
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Noguchi Kazuyoshi
Central Research Laboratories Ajinomoto Co. Inc.
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