Diversity within the Microcystin Biosynthetic Gene Clusters among the Genus Microcystis

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概要

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• The principle organization of microcystin biosynthesis (mcy) gene clusters is characterized in three genera among the microcystin-producing cyanobacteria. To examine the conservation of the mcy gene clusters among the genus Microcystis, internal non-coding regions of the cluster in 21 strains including M. aeruginosa, M. viridis, and M. wesenbergii, were amplified by PCR using specific primer sets and sequenced. PCR-based analysis revealed a completely conserved organization of the bidirectional cluster structure, mcyABC and mcyDEFGHIJ, among all toxic and some microcystin-negative strains. Furthermore, all mcy gene clusters were flanked by a gene with homology to dnaN adjacent to mcyJ and by uma1 with no-homology to any known genes at the 3'-end of mcyC. Transposase homolog genes were observed in a non-coding region between dnaN and mcyJ in three microcystin-producing Microcystis strains. Sequence analysis of a region adjacent to uma1 in mcy-negative strains revealed two types of gene constitution, dnaN-uma1 and the hypothetical protein gene-uma1. PCR-based analyses can be used to assess the identification of microcystin-producer strains in Microcystis spp.

著者

• NISHIZAWA TOMOYASU

  Ibaraki University College of Agriculture

• HARADA Ken-ichi

  Faculty of Pharmacy, Meijo University

• SHIRAI Makoto

  Laboratory of Molecular Genetics, Collage of Agriculture, Ibaraki University

• ASAYAMA Munehiko

  Laboratory of Molecular Genetics, Collage of Agriculture, Ibaraki University

• SHIRAI Makoto

  Ibaraki Univ.

• Harada Ken-ichi

  Laboratory Of Environmental Sciences Faculty Of Pharmacy Meijo University

• Shirai Makoto

  Coll. Of Agriculture Ibaraki Univ.

• Shirai Makoto

  Division Of Biotechnology School Of Agriculture Ibaraki University

• Shirai Makoto

  Dept. Biotec. Fac. Agric. Ibaraki Univ.

• Asayama M

  Coll. Of Agriculture Ibaraki Univ.

• Asayama Munehiko

  Laboratory Of Molecular Genetics College Of Agriculture Ibaraki University

• NISHIZAWA TOMOYASU

  Laboratory of Molecular Genetics, College of Agriculture, Ibaraki University

• NISHIZAWA AKITO

  Laboratory of Molecular Genetics, College of Agriculture, Ibaraki University

• NISHIZAWA TOMOYASU

  Department of Bioresource Science, Ibaraki University College of Agriculture

• Nishizawa Akito

  Laboratory Of Molecular Genetics College Of Agriculture Ibaraki University

• Nishizawa Tomoyasu

  Department Of Bioresource Science Ibaraki University College Of Agriculture

• Shirai Makoto

  Laboratory Of Molecular Genetics School Of Agriculture Ibaraki University

• Shirai Makoto

  Laboratory Of Molecular Genetics College Of Agriculture Ibaraki University

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