Light-responsive psbA Transcription Requires the - 35 Hexamer in the Promoter and Its Proximal Upstream Element, UPE, in Cyanobacteria(Biochemistry & Molecular Biology)

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概要

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• We characterized the function of the - 35 hexamer in the promoter and an element just upstream, UPE, in the expression in a unicellular cyanobacterium, Microcystis aeruginosa K-81, of the light-responsive gene psbA2, which encodes a reaction center key protein for photosynthesis. A series of mutants with mutations at the -35 hexamer (-35 to -30) and a novel conserved upstream element (UPE : -45 to -36, +1 referring to the transcription start point) were constructed. Expression of the mutants was examined in vivo and in vitro by analyses using a β-galactosidase assay, primer extension, and a DNA-mobility shift assay with RNA polymerases. Results indicated that the - 35 hexamer and its proximal UPE act as effective cis-elements for the lightresponsive and/or basal transcription, respectively. A model of the 5'-upstream region with cis- and possible trans-acting factors is presented for the psbA regulatory system.

• 社団法人日本農芸化学会の論文
• 2003-06-23

著者

• SHIRAI Makoto

  Laboratory of Molecular Genetics, Collage of Agriculture, Ibaraki University

• ASAYAMA Munehiko

  Laboratory of Molecular Genetics, Collage of Agriculture, Ibaraki University

• Asayama M

  Coll. Of Agriculture Ibaraki Univ.

• Asayama Munehiko

  Laboratory Of Molecular Genetics College Of Agriculture Ibaraki University

• ITO Yoko

  Laboratory of Molecular Genetics, School of Agriculture, Ibaraki University

• Ito Yoko

  Laboratory Of Molecular Genetics School Of Agriculture Ibaraki University

• Shirai Makoto

  Laboratory Of Molecular Genetics School Of Agriculture Ibaraki University

• Shirai Makoto

  Laboratory Of Molecular Genetics College Of Agriculture Ibaraki University

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