Isolation and Molecular Characterization of a Multicellular Cyanobacterium, Limnothrix/Pseudanabaena sp. Strain ARRG5-3

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概要

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• A cyanobacterium, semi-filamentous multicellular strain ABRG5-3, was isolated and its unique nature was characterized. This axenic strain formed colonies and was motile on an agarose plate. The 16S rRNA gene of ABRG5-3 exhibited similarities to those of the Limnothrix and Pseudanabaena strains, which are known as filamentous and nonheterocystous cyanobacteria. Peaks in absorbance for the accumulation of chlorophyll a, phycocyanin, and phycoerythrin were observed in the cell extract. Natural separation of the pigments occurred in the supernatant of the autolysed cells. The cell lysis was promoted by osmotic shocks and lysozyme treatments. Chlorophyll a and total DNA were abundantly recovered from the cells. Analysis of the restriction-modification system for genomic DNA revealed novel diversity. Moreover, we made a successful attempt to create antibiotic-resistant strains by conjugation with a foreign plasmid, which indicates that strain ABRG5-3 is transformable.

• 社団法人 日本農芸化学会の論文
• 2010-09-23

著者

• NISHIZAWA TOMOYASU

  Ibaraki University College of Agriculture

• KOMATSUZAKI MASAKAZU

  Ibaraki University College of Agriculture

• OHTA HIROYUKI

  Ibaraki University College of Agriculture

• Ohta Hiroyuki

  Ibaraki Univ. Coll. Of Agriculture

• Ohta Hiroyuki

  College of Agriculture, Ibaraki University

• SHIRAI Makoto

  Ibaraki Univ.

• Shirai Makoto

  Coll. Of Agriculture Ibaraki Univ.

• Shirai Makoto

  Division Of Biotechnology School Of Agriculture Ibaraki University

• Shirai Makoto

  Dept. Biotec. Fac. Agric. Ibaraki Univ.

• Asayama M

  Coll. Of Agriculture Ibaraki Univ.

• NISHIZAWA TOMOYASU

  Department of Bioresource Science, Ibaraki University College of Agriculture

• NISHIZAWA TOMOYASU

  College of Agriculture, Ibaraki University

• KOMATSUZAKI MASAKAZU

  College of Agriculture, Ibaraki University

• Miura Takamasa

  Coll. Of Agriculture Ibaraki Univ.

• Nishizawa Tomoyasu

  Department Of Bioresource Science Ibaraki University College Of Agriculture

• Takanezawa Akira

  College Of Agriculture Ibaraki University

• Watanabe Yuko

  College Of Agriculture Ibaraki University

• Shirai Makoto

  College Of Agriculture Ibaraki University

• HANAMI Tomoyo

  College of Agriculture, Ibaraki University

• HIRANO Eriko

  College of Agriculture, Ibaraki University

• MIURA Takamasa

  College of Agriculture, Ibaraki University

• ASAYAMA Munehiko

  College of Agriculture, Ibaraki University

• Ohta Hiroyuki

  College Of Agriculture Ibaraki University

• Miura Takamasa

  College Of Agriculture Ibaraki University

• Hirano Eriko

  College Of Agriculture Ibaraki University

• Hanami Tomoyo

  College Of Agriculture Ibaraki University

• Ohta Hiroyuki

  College Of Agr. Ibaraki Univ.

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