Phylogenetic and Functional Diversity of Denitrifying Bacteria Isolated from Various Rice Paddy and Rice-Soybean Rotation Fields
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概要
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Denitrifiers can produce and consume nitrous oxide (N2O). While little N2O is emitted from rice paddy soil, the same soil produces N2O when the land is drained and used for upland crop cultivation. In this study, we collected soils from two types of fields each at three locations in Japan; one type of field had been used for continuous cultivation of rice and the other for rotational cultivation of rice and soybean. Active denitrifiers were isolated from these soils using a functional single-cell isolation method, and their taxonomy and denitrifying properties were examined. A total of 110 denitrifiers were obtained, including those previously detected by a culture-independent analysis. Strains belonging to the genus Pseudogulbenkiania were dominant at all locations, suggesting that Pseudogulbenkiania denitrifiers are ubiquitous in various rice paddy soils. Potential denitrifying activity was similar among the strains, regardless of the differences in taxonomic position and soil of origin. However, relative amounts of N2 in denitrification end products varied among strains isolated from different locations. Our results also showed that crop rotation had minimal impact on the functional diversity of the denitrifying strains. These results indicate that soil and other environmental factors, excluding cropping systems, could select for N2-producing denitrifiers.
著者
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ISHII SATOSHI
Department of Soil, Water, and Climate, University of Minnesota
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OTSUKA SHIGETO
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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SENOO KEISHI
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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NISHIZAWA TOMOYASU
Ibaraki University College of Agriculture
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Senoo Keishi
Department Of Agricultual Chemistry The University Of Tokyo
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Otsuka Shigeto
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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TAGO Kanako
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Ishii Satoshi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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NISHIZAWA TOMOYASU
Department of Bioresource Science, Ibaraki University College of Agriculture
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Nishizawa Tomoyasu
Department Of Bioresource Science Ibaraki University College Of Agriculture
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Ishii Satoshi
Department Of Agricultural And Life Sciences Obihiro University Of Agriculture And Veterinary Medici
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Tago Kanako
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Nishizawa Tomoyasu
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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