Factors Controlling Long-Term Survival and Growth of Naturalized Escherichia coli Populations in Temperate Field Soils
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概要
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While Escherichia coli is widely used as an indicator of fecal contamination of waterways, recent studies suggest that this bacterium may become "naturalized" to soils. In this study, we investigated the survival and growth of naturalized E. coli in temperate soil in northern Minnesota. A spontaneously-occurring, antibiotic resistant E. coli strain, KS7-NR, was added to a field site at 103 cells (g soil)-1. The survival and growth of E. coli KS7-NR were followed from June to October, by using colony counts on agar plates supplemented with antibiotics, and by using quantitative PCR (qPCR) with strain-specific primers developed based on suppressive subtractive hybridization. Both plate count and qPCR analyses indicated that E. coli KS7-NR survived for more than 2 months in the field soil. Laboratory experiments showed that soil temperature, moisture, nutrients, and the presence of other soil organisms influenced growth and survival of E. coli KS7-NR in soil. Moreover, our results indicated that summer drought conditions that occurred during the field study likely limited in situ growth of E. coli at the field site. Taken together, these results suggest that E. coli strains can survive long-term and grow in soils, provided that abiotic factors are within their tolerance limits.
- 2010-03-01
著者
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ISHII SATOSHI
Department of Soil, Water, and Climate, University of Minnesota
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YAN TAO
BioTechnology Institute, University of Minnesota
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VU HUNG
Department of Soil, Water, and Climate, University of Minnesota
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HANSEN DENNIS
Department of Biology, University of Minnesota-Duluth
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HICKS RANDALL
Department of Biology, University of Minnesota-Duluth
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SADOWSKY MICHAEL
Department of Soil, Water, and Climate, University of Minnesota
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Yan Tao
Biotechnology Institute University Of Minnesota
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Yan Tao
Biotech. Inst. Akita Pref. Univ.
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Vu Hung
Department Of Soil Water And Climate University Of Minnesota
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Hicks Randall
Department Of Biology University Of Minnesota-duluth
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Hansen Dennis
Department Of Biology University Of Minnesota-duluth
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Sadowsky Michael
Department Of Soil Water And Climate University Of Minnesota
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Ishii Satoshi
Department Of Agricultural And Life Sciences Obihiro University Of Agriculture And Veterinary Medici
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Ishii Satoshi
Department Of Soil Water And Climate University Of Minnesota
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Sadowsky Michael
Department Of Soil Water And Climate And Biotechnology Institute University Of Minnesota
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