Genetic Transformation System for Members of the Genera, Sphingomonas, Sphingobium, Novosphingobium and Sphingopyxis
スポンサーリンク
概要
- 論文の詳細を見る
This paper describes a plasmid transformation system that permits genetic manipulation of the genus Sphingomonas. A cryptic indigenous plasmid, pAMI-1, of 10kb from Sphingobium amiense was isolated and characterized. Nucleotide sequencing revealed that pAMI-1 contains five open reading frames, which are predicted to encode proteins associated with integration, recombination, conjugation and replication. Escherichia coli-S. amiense shuttle vectors were successfully introduced into Sphingomonas, Sphingobium, Novosphingobium and Sphingopyxis strains by electroporation. The copy number of the shuttle vector was estimated to be 1-2 per chromosome in the Sphingobium yanoikuyae cell.
- 日本微生物生態学会の論文
著者
-
OHTA HIROYUKI
Ibaraki University College of Agriculture
-
Kurusu Yasurou
Ibaraki University College Of Agriculture
-
Ohta Hiroyuki
Ibaraki Univ.
-
Saito Miyuki
Ibaraki University College Of Agriculture
-
IKUNAGA YOKO
Ibaraki University College of Agriculture
関連論文
- Molecular Characterization of Fungal Communities in Non-Tilled, Cover-Cropped Upland Rice Field Soils
- Optimization of a Methylation Procedure to Obtain Chloroform-Soluble Humic Acids
- Soil Chemical Properties and Distribution of Sclerotium Grains in Forest Soils, Harz Mts., Germany(Soil Biology)
- Aluminum Concentrations in Sclerotia from a Buried Humic Horizon of Volcanic Ash Soils in Mt.Myoko, Central Japan
- Humification of Three Plant Residues with Sakurajima Volcanic Ash as Influenced by Phosphate Addition and Low pH Condition
- Occurrence of Smectite on the Pumice Surface Derived from Taal Volcano in the Phillppines
- Nitrogenase Activity (Acetylene Reduction) of an Iron-Oxidizing Leptospirillum Strain Cultured as a Pioneer Microbe from a Recent Volcanic Deposit on Miyake-Jima, Japan
- P-14 Microbial population and activity in volcanic deposits with different succession stage of vegetation in Miyake-jima Island
- Atmospheric CO and Hydrogen Uptake and CO Oxidizer Phylogeny for Miyake-jima, Japan Volcanic Deposits
- 08-143 種々の窒素化合物培地におけるMetarhizium strain CY-14の亜酸化窒素生成(生理/代謝/増殖,研究発表)
- Archaeal Diversity of Upland Rice Field Soils Assessed by the Terminal Restriction Fragment Length Polymorphism Method Combined with Real Time Quantitative-PCR and a Clone Library Analysis
- Relationships between Fungal Biomass and Nitrous Oxide Emission in Upland Rice Soils under No Tillage and Cover Cropping Systems
- Characterization of Herbaspirillum- and Limnobacter-Related Strains Isolated from Young Volcanic Deposits in Miyake-Jima Island, Japan
- PB-76 Isolation and culture-based and culture-independent analysis of iron-oxidizing bacteria in the Miyake-jima Island volcanic deposits(Community structure,Poster presentation B)
- PB-74 Molecular diversity of nifH gene in the Miyake-jima volcanic deposits ranging from 6 to >800 years old(Community structure,Poster presentation B)
- PB-50 Carbonyl sulfide (COS)-degrading activity and phylogenetic identity of the bacterial isolates from the MPN for counting COS-degrading microbes in soils(Matter cycling,Poster presentation B)
- PA-29 Diversity of soil microbial communities assessed by PCR-amplified T-RFLP analysis : effects of tillage treatments(Agricultural ecosystem,Poster presentation A)
- PA-27 Microbial nitrous oxide emission from agricultural soils under different nitrogen application(Agricultural ecosystem,Poster presentation A)
- 3-05 Detection and characterization of endosymbiotic bacteria in the denitrifying fungi(Symbiosis/Cell to cell communication,Oral presentation)
- 2-14 Characterization of Limnobacter, Herbaspirillum and Paracoccus strains isolated from volcanic deposits in Miyake-jima Island(Taxonomy/Phylogeny,Oral presentation)
- 2-05 Isolation and characterization of Thallium-accumulating soil bacteria(Physiology/Growth,Oral presentation)
- 1-24 Effect of copper nonylphenol sulfonate on soil microbial communities(Community structure 2,Oral presentation)
- 1-13 Denitrification activities of fungal isolates from agricultural soils.(Agricultural ecosystem,Oral presentaion)
- Isolation and Characterization of Thallium-tolerant Bacteria from Heavy Metal-polluted River Sediment and Non-polluted Soils
- PB-52 Predominance of Limnobacter and Herbaspirillum in recent Miyake-jima volcanic deposits(Microbial community analysis,Session B,(1) Poster Presentation)
- Isolation of Octylphenol Polyethoxylate-Degrading Soil Bacteria : a Long-Term Soil Column Study
- PB-45 Bacterial community structures of the volcanic ash (2000-y) and scoria deposits (1874- and 1962-y) in Miyake-jima Island(CLASSIFICATION/PHYLOGENETIC ANALYSIS,Session B,(1) Poster presentations)
- Genetic Transformation System for Members of the Genera, Sphingomonas, Sphingobium, Novosphingobium and Sphingopyxis
- Isolation and Molecular Characterization of a Multicellular Cyanobacterium, Limnothrix/Pseudanabaena sp. Strain ARRG5-3
- Detection of Betaproteobacteria inside the Mycelium of the Fungus Mortierella elongata
- PB-45 Utilization of phenolic acids by soil bacteria(Metabolism/Chemical activity,Poster presentation B)
- PA-61 Growth characteristics of Pseudomonas aeruginosa strain under the clinorotation(Physiology/Growth,Poster presentation A)
- B-06 Enrichment of soil bacteria able to degrade octylphenol polyethoxylates in a soil percolation column(BIOREMEDIATION,(2) Oral presentation)
- P-051 Degradation of alkylphenol polyethoxylates by soil bacteria : a model study using soil percolation columns
- Combined Analyses of Bacterial, Fungal and Nematode Communities in Andosolic Agricultural Soils in Japan
- The characterization of Herbaspirillum- like strains isolated from recent volcanic deposits in Miyake-jima Island(The annal Meeting of the Society in 2007)
- Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil
- Complete Genome Sequence of Bradyrhizobium sp. S23321 : Insights into Symbiosis Evolution in Soil Oligotrophs
- Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil