Production of Transgenic Japonica Rice (Oryza sativa) Cultivar, Taichung 65, by the Agrobacterium-Mediated Method
スポンサーリンク
概要
- 論文の詳細を見る
Transgenic rice plants of Taiwanese japonica rice cultivar, Taichung 65, were obtained by co-cultivating scutellum calli with an Agrobacterium tumefaciens strain, EHA101, that carried a binary vector harboring the luciferase (Luc) gene driven by the CaMV35S promoter. The transformation efficiency of Taichung 65 was similar to that obtained by the methods routinely used for a transformable cultivar, Notohikari. There was no correlation between the length of the culture period (21 to 30 days) and the transient transformation efficiency in Taichung 65 and in Notohikari. In the T0- and T1-generations, the transgenes were integrated and stably expressed, indicating that the transgene was inherited to the next generation. The copy number of integrated transgene varied from one to three in the T1-transformants, which was confirmed by Southern blot analysis. Moreover, approximately 60% of the T1-transformants of Taichung 65 showed the LUC-positive phenotype. These results suggest that, in addition to Notohikari, Taichung 65 is a practical, transformable cultivar.
- 日本植物細胞分子生物学会の論文
- 2001-12-01
著者
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Matsuda Osamu
Department of Cardiology, Toyohashi heart center
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Yara Asanori
Department Of Biology Kyushu University
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KUSUMI Kensuke
Department of Biology, Faculty of Science, Kyushu University
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IBA Koh
Department of Biology, Faculty of Science, Kyushu University
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OTANI Motoyasu
Research Institute of Agricultural Resources, Ishikawa Agricultural College
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SHIMADA Takiko
Research Institute of Agricultural Resources, Ishikawa Agricultural College
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Iba Koh
Department Of Biology Kyushu University
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Iba Koh
Department Of Biology Faculty Of Science Kyushu University
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Shimada T
Research Institute Of Agricultural Resources Ishikawa Agricultural College
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Otani M
Ishikawa Agriculture Coll. Ishikawa Jpn
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Otani Motoyasu
Research Institute Of Agricultural Resources Ishikawa Agricultural College
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Kusumi K
Department Of Biology Faculty Of Science Kyushu University
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Kusumi Kensuke
Department Of Biology Kyushu University
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Kusumi Kensuke
Department Of Biology Faculty Of Science Kyushu University
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Kusumi Kensuke
Department Of Biology Faculty Of Sciences Kyushu University
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Shimada Takiko
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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Shimada T
Ishikawa Agricultural Coll. Ishikawa Jpn
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Iba Koh
Department Of Biology Faculty Of Sciences Kyushu University
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Matsuda O
Hiroshima Univ. Higashi‐hiroshima Jpn
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Matsuda O
Research Center Sony Corporation
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Yara Asanori
Department Of Biology Faculty Of Sciences Kyushu University
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Matsuda Osamu
Department Of Applied Physics Faculty Of Engineering Hokkaido University
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Matsuda Osamu
Sony Corporation Research Center
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Otani Motoyasu
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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