Thermodynamics of Malate Transport across the Tonoplast of Leaf Cells of CAM Plants : MEMBRANES AND BIOENERGETICS
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概要
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The electrochemical potential difference for each dissociation state of malic acid across the tonoplast of leaf cells was examined in two CAM plants, Graptopetalum paraguayense and Kalanchoe daigremontiana. The concentration of malic acid in each dissociation state was estimated from an analysis of pH and concentrations of ionic species that included calcium, malate and isocitrate. The vacuoles contained 30-40 mM isocitrate and 50-70 mM calcium in G.paraguayense, and 20-30 mM isocitrate and 70-100 mM calcium in K. daigremontiana. For the calculation of the pattern of dissociation of malic acid, the formation of chelates of calcium with malate and isocitrate, which have different stability constants depending on the dissociation of the acids, were also taken into consideration. The vacuolar concentrations of the divalently dissociated form of malic acid (mal^<2->) were 4-7 mM and 1-3 mM in G.paraguayense and in K. daigremontiana, respectively. To obtain information about the cytoplasmic concentration of malate, the apparent inhibition constant for malate of phosphoenolpyruvate carboxylase was measured. It was about 330 μM in the dark period and 60 μM in the light period. Considering an inside-positive membrane potential, we conclude that mal^<2-> can be taken up passively into the vacuole during the dark period and can be released passively from the vacuole during the light period. Two types of channel (the "SV type" channel and a novel "MU-type" channel) which we found recently in G. paraguayense [Iwasaki et al. (1992) Plant Physiol. 98: 1494] are probably involved in the uptake and the release of malate in the diurnal CAM rhythm. The existence of a large pH-buffering capacity due to isocitric acid in the vacuole allows the accumulation of a large amount of malic acid during the diurnal CAM rhythm.
- 日本植物生理学会の論文
著者
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KUSUMI Kensuke
Department of Biology, Faculty of Science, Kyushu University
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Kusumi K
Department Of Biology Faculty Of Science Kyushu University
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Kusumi Kensuke
Department Of Biology Kyushu University
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Kusumi Kensuke
Department Of Biology Faculty Of Science Kyushu University
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Kusumi Kensuke
Minase Research Institute Ono Pharmaceutical Co. Ltd.
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Kusumi Kensuke
Department Of Biology Faculty Of Sciences Kyushu University
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ARATA Hiroyuki
Department of Biology, Graduate School of Sciences, Kyushu University
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Nishimura Mitsuo
Department of Biology, Faculty of Science, Kyushu University
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Nishimura M
Dept. Cell Biol. Nat. Inst. Basic Biol.
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Nishimura Mitsuo
Departmcnt Of Biology Faculty Of Science Kyushu University
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Arata H
Department Of Biology Graduate School Of Sciences Kyushu University
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Iwasaki I
Akita Prefectural Univ. Akita Jpn
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Iwasaki Ikuko
Department Of Bio-resource Science Akita Prefectural University
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