Co-integration, co-expression and co-segregation of an unlinked selectable marker gene and NtFAD3 gene in transgenic rice plants produced by particle bombardment

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概要

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• Using the scutellar tissue of rice mature embryos as a target tissue, a selectable marker gene, bar, and an unselectable gene, fatty acid desaturase gene from tobacco (NtFAD3), on separate plasmids, were introduced by particle bombardment. Co-integration, co-expression and inheritance of these genes were analyzed as well as seed fertility of the transgenic plants. Twenty-three out of 32 bialaphos-resistant plants integrated the NtFAD3 gene, which was confirmed by Southern-blot analysis of R0 plants, and showed one to more than 20 hybridizing bands of exogenous DNA, indicating a 72% (23/32) co-integration frequency. However, the frequency of the transgenic plants containing the 1.4-kb fragment of NtFAD3 gene was 34% (11/32). Northern-blot analysis revealed that seven out of ten fertile transgenic rice plants which had a 1.4-kb fragment of NtFAD3 cDNA expressed NtFAD3 mRNA. The NtFAD3 gene under the control of CaMV35S promoter stably expressed in the transgenic rice plants and modified the proportions of linoleic acid (18:2) and linolenic acid (18:3) in fatty acids; the content of 18:2 decreased and that of 18:3 increased. Fourteen out of 32 (44%) transgenic plants set seeds and 18 (56%) showed low fertility or sterility. Molecular analysis of the selfed progeny indicated that all copies in almost all R0 plants were inherited as a single dominant hemizygous locus.

• 日本遺伝学会の論文

著者

• IBA Koh

  Department of Biology, Faculty of Science, Kyushu University

• OTANI Motoyasu

  Research Institute of Agricultural Resources, Ishikawa Agricultural College

• SHIMADA Takiko

  Research Institute of Agricultural Resources, Ishikawa Agricultural College

• Iba Koh

  Department Of Biology Kyushu University

• Iba Koh

  Department Of Biology Faculty Of Science Kyushu University

• Wakita Y

  Research Institute Of Agricultural Resources Ishikawa Agricultural College

• Shimada T

  Research Institute Of Agricultural Resources Ishikawa Agricultural College

• Otani M

  Ishikawa Agriculture Coll. Ishikawa Jpn

• Otani Motoyasu

  Research Institute Of Agricultural Resources Ishikawa Agricultural College

• Shimada T

  Esearch Institute Of Agricultural Resources Ishikawa Agricultural College

• WAKITA Yohichi

  Research Institute of Agricultural Resources, Ishikawa Agricultural College

• Wakita Y

  Cell Manipulation Laboratory Bio-resources Technology Division Forestry And Forest Products Research

• Wakita Yohichi

  Research Institute Of Agricultural Resources Ishikawa Agricultural College

• Shimada Takiko

  Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University

• Iba Koh

  Department Of Biology Faculty Of Sciences Kyushu University

• WAKITA Yoichi

  Research Institute of Agricultural Resources, Ishikawa Agricultural College

• Otani Motoyasu

  Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University

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