Doubled haploid plant production of transgenic rice (Oryza sativa L.) using anther culture
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概要
- 論文の詳細を見る
Anthers of transgenic rice plants with two foreign genes were cultured to obtain doubled haploid plants that possessed the introduced foreign genes homozygously. Anther culture response varied with the transgenic line. Green plantlets were regenerated from the microspore-derived calli, and 5.9–88.9% of the regenerated plants set numerous seeds in a greenhouse. The haploid or doubled haploid green plants were confirmed to have the foreign genes by Southern hybridization analysis. They had both or no foreign genes, and no plants with heterozygosity in transgenes were observed. The ratio of the plants with the transgenes homozygously to those without the transgenes varied with the donor transgenic lines.
- 日本植物細胞分子生物学会の論文
- 2005-06-01
著者
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OTANI Motoyasu
Research Institute of Agricultural Resources, Ishikawa Agricultural College
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SHIMADA Takiko
Research Institute of Agricultural Resources, Ishikawa Agricultural College
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Wakita Y
Research Institute Of Agricultural Resources Ishikawa Agricultural College
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Shimada T
Research Institute Of Agricultural Resources Ishikawa Agricultural College
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Otani M
Ishikawa Agriculture Coll. Ishikawa Jpn
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Otani Motoyasu
Research Institute Of Agricultural Resources Ishikawa Agricultural College
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Shimada T
Esearch Institute Of Agricultural Resources Ishikawa Agricultural College
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Wakita Y
Cell Manipulation Laboratory Bio-resources Technology Division Forestry And Forest Products Research
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Wakita Yohichi
Research Institute Of Agricultural Resources Ishikawa Agricultural College
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Shimada Takiko
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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WAKITA Yoichi
Research Institute of Agricultural Resources, Ishikawa Agricultural College
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Otani Motoyasu
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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