Effects of Calcium Antagonists on the Nitrergic Nerve Function in Canine Corpus Cavernosum

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Effects of calcium antagonists on nitrergic nerve function were examined in the isolated canine corpus cavernosum. In the cavernous strips precontracted with phenylephrine, transmural electrical stimulation elicited frequency-dependent (2 – 5 Hz) relaxations that were abolished by NG-nitro-<sc>L</sc>-arginine (10−5 M), a nitric oxide (NO) synthase inhibitor; 1H[1,2,4]oxadiazole[4,3-a]quinoxalin-1-one (ODQ, 10−6 M), a soluble guanylate cyclase inhibitor; and tetrodotoxin (3 × 10−7 M). The relaxations were not affected by treatment with nifedipine or nicardipine (10−8 – 10 −6 M), L-type specific calcium channel inhibitors, but were significantly inhibited by amlodipine or cilnidipine, inhibitors of L- plus N-type calcium channels, in a concentration-related manner (10−7 – 10 −6 M). All of the inhibitors used did not affect the relaxations induced by exogenous NO (acidifed NaNO2). These findings suggest that N-type, but not L-type, calcium channels are responsible for increasing cytosolic free calcium, a prerequisite for the synthesis of NO, in the nitrergic dilator nerves innervating the corpus cavernosum.
社団法人日本薬理学会の論文
2001-11-01