Protein Crystallization Using a High-Repetitive Low-Power Laser Beam Under Tightly Focused Condition
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概要
- 論文の詳細を見る
The feasibility of a simple laser irradiation scheme that utilizes a low-power laser beam emitted directly from a femtosecond laser oscillator was studied using lysozyme as an example for promoting protein crystallization. The various irradiation intensities required for crystallization promotion were surveyed by the hanging drop crystallization method under a tightly focused condition. The optimal irradiation condition was found to be 10 GW/cm2, and the rate of lysozyme crystallization increased five times compared with that for the nonirradiated sample. On the other hand, when an intensity of 45 GW/cm2 was applied, polycrystals were generated; the rate of crystallization was low with irradiation less than 5 GW/cm2.
- Japan Society of Applied Physicsの論文
- 2004-07-15
著者
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Tashiro Hideo
Riken
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Watanabe Shu
RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
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Noda Kohki
RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
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Nagasaka Shinobu
RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
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Tashiro Hideo
RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
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