DNA Polymerase .BETA.s from Liver and Testes of Cherry Salmon, Oncorhynchus masou: Purification and Characterization of DNA Polymerase .BETA.s with Acidic Isoelectric Points.
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概要
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DNA polymerase βs from cherry salmon, Oncorhynchus masou, liver and testes were purified to near homogeneity, and no substantial differences between the enzymes were observed. The molecular weight of both enzymes, determined by SDS-polyacrylamide gel electrophoresis, was 39, 000. The amino acid sequences of the N-terminus of the liver and testes enzymes were determined and compared with that of the rat enzyme. Of the N-terminal 30 amino acid residues of salmon liver DNA polymerase β, 21 (70%) were identical to those of the rat enzyme sequence. However, unlike most eukaryotic DNA polymerase βs, the isoelectric points (pIs) of the DNA polymerase βs from salmon liver and testes were both estimated to be 6.2, which is significantly different from the alkaline isoelectric points (pI=8.5-9.5) established for other highly purified vertebrate DNA polymerase βs. The cherry salmon DNA polymerase βs were still active at below 10°C, compared with the rat enzyme.
- 社団法人 日本生化学会の論文
著者
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Hirai Toshiaki
Department Of Biological Science Teikyo University Science And Technology
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Saneyoshi Mineo
Department Of Biological Science Teikyo University Of Science And Technology
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Yoshikuni Michiyasu
Department Of Developmental Biology National Institute For Basic Biology
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Masaki Junya
Department Of Biological Sciences The Nishi-tokyo University
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Yamaguchi Toyofumi
Department Of Biological Sciences The Nishi-tokyo University
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Takahashi Keiko
Department Of Applied Chemistry Faculty Of Engineering Tokyo Institute Of Polytechnics
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Nishimura Shigeko
Shizuoka Higashi Hospital, National Epilepsy Center
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