ROLE OF CpG ODN IN CONCANAVALIN A-INDUCED HEPATITIS IN MICE
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概要
- 論文の詳細を見る
Objective: To investigate the effects of an intradermal injection of oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs on concanavalin A (Con A)-induced hepatitis, an experimental model of immune-mediated hepatitis.Methods: Con A was injected intravenously into Balb/c mice. Twelve hours after Con A challenge, blood and liver samples were obtained. CpG ODN was injected intradermally 48 hours before Con A challenge. The extent of liver injury was assessed by determining serum alanine transaminase (ALT) and by liver histology. Serum levels of cytokines, including interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-4 and IL-5, were measured by enzyme-linked immunosorbent assay.Results: Co-administration of Con A and CpG ODN significantly increased serum ALT in mice compared with that in the case of administration of Con A alone (10,268±4,654 and 1,140±832 IU/l, respectively, p<0.05). In liver histology, mice treated with CpG ODN and Con A showed more extensive midzonal necrosis than did mice treated with Con A alone. These mice also showed significant increases in serum TNF-α and IFN-γ and decrease in serum IL-5.Conclusions: The results indicate that CpG ODNs aggravate Con A-induced hepatitis by stimulating the production of T-helper-1 (Th1) cytokines, TNF-α and IFN-γ, suggesting that bacterial DNA that contains unmethylated CpG motifs may contribute to the exacerbation of immune-mediated liver injury.
- 福島医学会の論文
著者
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KOBAYASHI Hiroko
Department of Internal Medicine II, Fukushima Medical University School of Medicine
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Sato Yukio
Department Of Advanced Materials Science Graduate School Of Frontier Science The University Of Tokyo
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Abe Kazumichi
Department Of Gastroenterology And Rheumatology Fukushima Medical University School Of Medicine
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Kobayashi Hiroko
Department Of Gastroenterology And Rheumatology Fukushima Medical University School Of Medicine
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Takahashi Atsushi
Departmant Of Surgery(i) Kanazawa University School Of Medicine
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OHIRA HIROMASA
Department of Gastroenterology and Rheumatology
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Takahashi Atsushi
Department of Internal Medicine II, Fukushima Medical University School of Medicine
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SAITO HIRONOBU
Department of Internal Medicine II, Fukushima Medical University School of Medicine
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Saito Hironobu
Department of Gastroenterology and Rheumatology, Fukushima Medical University School of Medicine, Japan
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RAI TSUYOSHI
Department of Internal Medicine II, Fukushima Medical University School of Medicine
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Kobayashi Hiroko
Department of Cardiovascular Disease Research, Eisai Tsukuba Research Laboratories
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