Effect of Overexpression of the Brain-Specific Na+/Ca2+ Exchanger Splice Variant NCX1.5 on NO Cytotoxicity in HEK293 Cells
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概要
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Nitric oxide (NO) induces cytotoxicity in neuronal and glial cells via activation of the Na+/Ca2+ exchanger (NCX). This study examined the role of the predominant brain-specific NCX splice variant NCX1.5 in NO-induced cytotoxicity in the HEK293 cell expression system. Cells were transfected with the plasmid construct pcDNA3.1/V5-His containing full-length rat NCX1.5 cDNA. There was no difference in the cytotoxic effects of the NO donors sodium nitroprusside and S-nitroso-N-acetylpenicillamine between control and transfected cells. These results suggest that NO cytotoxicity is not dependent on NCX1.5.
- 公益社団法人 日本薬理学会の論文
著者
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Matsuda Toshio
Laboratory Of Medicinal Pharmacology Graduate School Of Pharmaceutical Sciences Osaka University
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Ago Yukio
Laboratory Of Medicinal Pharmacology Graduate School Of Pharmaceutical Sciences Osaka University
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Kawanai Takuya
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushi
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Takuma Kazuhiro
Laboratory Of Medicinal Pharmacology Graduate School Of Pharmaceutical Sciences Osaka University
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Watanabe Ryo
Laboratory of Pharmaceutical Organic Chemistry, Osaka University of Pharmaceutical Sciences
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Matsuda Toshio
Laboratory of Medicinal Pharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Japan
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Ota Yuki
Laboratory of Medicinal Pharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Japan
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Nishimura Akira
Laboratory of Medicinal Pharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Japan
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Kawanai Takuya
Laboratory of Medicinal Pharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Japan
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Watanabe Ryo
Laboratory of Medicinal Pharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Japan
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- Effect of Overexpression of the Brain-Specific Na+/Ca2+ Exchanger Splice Variant NCX1.5 on NO Cytotoxicity in HEK293 Cells
- Effect of Overexpression of the Brain-Specific Na^+/Ca^ Exchanger Splice Variant NCX1.5 on NO Cytotoxicity in HEK293 Cells