Development of a Sandwich Enzyme-Linked Immunosorbent Assay to Detect and Measure Serum Levels of Canine Ferritin
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概要
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We established a homologous sandwich enzyme-linked immunosorbent assay (ELISA) to measure serum levels of canine ferritin. Our assay uses a rabbit anti-canine heart ferritin polyclonal antibody, and canine heart ferritin as a standard. Serum ferritin concentration in healthy dogs (n=163) was 789 ± 284 ng/ml (mean ± standard deviation), a value higher than reported previously. Confidence levels relating to repeatability, dilution, and recovery for this method were high. Therefore, we believe our developed sandwich ELISA will be effective in evaluating serum levels of canine ferritin.
著者
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Higuchi Sei-ichi
Department Of Small Animal Internal Medicine School Of Veterinary Medicine Kitasato University
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Hoshi Fumio
Department Of Small Animal Internal Medicine School Of Veterinary Medicine Kitasato University
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Hori Yasutomo
Department Of Internal Medicine School Of Veterinary Medicine & Animal Science Kitasato Universi
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CHIKAZAWA Seishiro
Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
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ORINO Koichi
Laboratory of Biochemistry, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
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KANAI Kazutaka
Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
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KANAI Kazutaka
Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
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Ito Naoyuki
Department of Chemical Engineering, Tokyo Institute of Technology
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WATANABE Kiyotaka
Laboratory of Biochemistry, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
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SATO Jun
Tomizawa Animal Hospital, 2–1–30 Tomizawa Taihaku-ku, Sendai, Miyagi 982–0032, Japan
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