Purification and Quantification of Lactoferrin in Equine Seminal Plasma

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概要

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• Lactoferrin with a molecular mass of 80 kDa was purified from equine seminal plasma by heparin-Agarose affinity chromatography and Sephacryl S-200 gel filtration. Purified lactoferrin was found to be highly homogeneous on the bases of its migration as a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and of the monospecificity of rabbit antibodies to the purified protein in immunoblotting of seminal plasma proteins. A sandwich enzyme-linked immunosorbent assay was developed for quantifying lactoferrin in equine seminal plasma. Seminal plasma lactoferrin concentrations in 23 normal stallions ranged from 42 to 453 μg/ml, with a mean value of 157 ± 118 μg/ml (S.D.).

著者

• Kikuchi Motohiro

  Laboratory Of Theriogenology School Of Veterinary Medicine Kitasato University

• Ohnami Yohji

  Laboratory Of Theriogenology School Of Veterinary Medicine Kitasato University

• Orino Koichi

  Laboratory Of Veterinary Biochemistry School Of Veterinary Medicine Kitasato University

• Watanabe Kiyotaka

  Laboratory Of Veterinary Biochemistry School Of Veterinary Medicine Kitasato University

• OHNAMI Yohji

  Laboratory of Theriogenology, School of Veterinary Medicine and Animal Sciences, Kitasato University

• INAGAKI Masami

  Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University

• ORINO Koichi

  Laboratory of Biochemistry, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan

• KIKUCHI Motohiro

  Laboratory of Theriogenology, School of Veterinary Medicine and Animal Sciences, Kitasato University

関連論文

• Purification and Quantification of Lactoferrin in Equine Seminal Plasma
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